4qaz

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==The crystal structure of the C-terminal domain of Ebola (Zaire) nucleoprotein==
==The crystal structure of the C-terminal domain of Ebola (Zaire) nucleoprotein==
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<StructureSection load='4qaz' size='340' side='right' caption='[[4qaz]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
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<StructureSection load='4qaz' size='340' side='right'caption='[[4qaz]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4qaz]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QAZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QAZ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4qaz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ebola_virus_-_Mayinga,_Zaire,_1976 Ebola virus - Mayinga, Zaire, 1976]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QAZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QAZ FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4qb0|4qb0]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qaz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qaz OCA], [http://pdbe.org/4qaz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qaz RCSB], [http://www.ebi.ac.uk/pdbsum/4qaz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qaz ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qaz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qaz OCA], [https://pdbe.org/4qaz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qaz RCSB], [https://www.ebi.ac.uk/pdbsum/4qaz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qaz ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/NCAP_EBOZM NCAP_EBOZM]] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases.
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[https://www.uniprot.org/uniprot/NCAP_EBOZM NCAP_EBOZM] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ebolavirus (EBOV) causes severe hemorrhagic fever with a mortality rate of up to 90%. EBOV is a member of the order Mononegavirales and, like other viruses in this taxonomic group, contains a negative-sense single-stranded (ss) RNA. The EBOV ssRNA encodes seven distinct proteins. One of them, the nucleoprotein (NP), is the most abundant viral protein in the infected cell and within the viral nucleocapsid. Like other EBOV proteins, NP is multifunctional. It is tightly associated with the viral genome and is essential for viral transcription, RNA replication, genome packaging and nucleocapsid assembly prior to membrane encapsulation. NP is unusual among the Mononegavirales in that it contains two distinct regions, or putative domains, the C-terminal of which shows no homology to any known proteins and is purported to be a hub for protein-protein interactions within the nucleocapsid. The atomic structure of NP remains unknown. Here, the boundaries of the N- and C-terminal domains of NP from Zaire EBOV are defined, it is shown that they can be expressed as highly stable recombinant proteins in Escherichia coli, and the atomic structure of the C-terminal domain (residues 641-739) derived from analysis of two distinct crystal forms at 1.98 and 1.75 A resolution is described. The structure reveals a novel tertiary fold that is distantly reminiscent of the beta-grasp architecture.
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The structure of the C-terminal domain of the Zaire ebolavirus nucleoprotein.,Dziubanska PJ, Derewenda U, Ellena JF, Engel DA, Derewenda ZS Acta Crystallogr D Biol Crystallogr. 2014 Sep 1;70(Pt 9):2420-9. doi:, 10.1107/S1399004714014710. Epub 2014 Aug 29. PMID:25195755<ref>PMID:25195755</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4qaz" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Nucleoprotein|Nucleoprotein]]
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*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Derewenda, U]]
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[[Category: Ebola virus - Mayinga, Zaire, 1976]]
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[[Category: Derewenda, Z S]]
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[[Category: Large Structures]]
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[[Category: Dziubanska, P J]]
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[[Category: Derewenda U]]
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[[Category: Ebola virus vp40]]
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[[Category: Derewenda ZS]]
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[[Category: New family]]
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[[Category: Dziubanska PJ]]
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[[Category: Viral protein]]
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Current revision

The crystal structure of the C-terminal domain of Ebola (Zaire) nucleoprotein

PDB ID 4qaz

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