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| | ==A236G Epi-isozizaene synthase: Complex with Mg, inorganic pyrophosphate and benzyl triethyl ammonium cation== | | ==A236G Epi-isozizaene synthase: Complex with Mg, inorganic pyrophosphate and benzyl triethyl ammonium cation== |
| - | <StructureSection load='4lz0' size='340' side='right' caption='[[4lz0]], [[Resolution|resolution]] 1.75Å' scene=''> | + | <StructureSection load='4lz0' size='340' side='right'caption='[[4lz0]], [[Resolution|resolution]] 1.75Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4lz0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LZ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LZ0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4lz0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LZ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LZ0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTM:N-BENZYL-N,N-DIETHYLETHANAMINIUM'>BTM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.754Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3kb9|3kb9]], [[3kbk|3kbk]], [[3lg5|3lg5]], [[3lgk|3lgk]], [[4ltv|4ltv]], [[4ltz|4ltz]], [[4luu|4luu]], [[4lxw|4lxw]], [[4lz3|4lz3]], [[4lzc|4lzc]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTM:N-BENZYL-N,N-DIETHYLETHANAMINIUM'>BTM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cyc1, SCO5222, SC7E4.19 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lz0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lz0 OCA], [https://pdbe.org/4lz0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lz0 RCSB], [https://www.ebi.ac.uk/pdbsum/4lz0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lz0 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Epi-isozizaene_synthase Epi-isozizaene synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.37 4.2.3.37] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lz0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lz0 OCA], [http://pdbe.org/4lz0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lz0 RCSB], [http://www.ebi.ac.uk/pdbsum/4lz0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lz0 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CYC1_STRCO CYC1_STRCO]] Catalyzes the cyclization of farnesyl diphosphate (FPP) to the sesquiterpene epi-isozizaene. | + | [https://www.uniprot.org/uniprot/CYC1_STRCO CYC1_STRCO] Catalyzes the cyclization of farnesyl diphosphate (FPP) to the sesquiterpene epi-isozizaene. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Epi-isozizaene synthase]] | + | [[Category: Large Structures]] |
| - | [[Category: Strco]]
| + | [[Category: Cane DE]] |
| - | [[Category: Cane, D E]] | + | [[Category: Chou W]] |
| - | [[Category: Chou, W]] | + | [[Category: Christianson DW]] |
| - | [[Category: Christianson, D W]] | + | [[Category: Harris G]] |
| - | [[Category: Harris, G]] | + | [[Category: Himmelberger JA]] |
| - | [[Category: Himmelberger, J A]] | + | [[Category: Li R]] |
| - | [[Category: Li, R]] | + | [[Category: Litwin K]] |
| - | [[Category: Litwin, K]] | + | |
| - | [[Category: Class i terpene cyclase]]
| + | |
| - | [[Category: Lyase]]
| + | |
| Structural highlights
Function
CYC1_STRCO Catalyzes the cyclization of farnesyl diphosphate (FPP) to the sesquiterpene epi-isozizaene.
Publication Abstract from PubMed
The class I terpenoid cyclase epi-isozizaene synthase (EIZS) utilizes the universal achiral isoprenoid substrate, farnesyl diphosphate, to generate epi-isozizaene as the predominant sesquiterpene cyclization product and at least five minor sesquiterpene products, making EIZS an ideal platform for the exploration of fidelity and promiscuity in a terpenoid cyclization reaction. The hydrophobic active site contour of EIZS serves as a template that enforces a single substrate conformation, and chaperones subsequently formed carbocation intermediates through a well-defined mechanistic sequence. Here, we have used the crystal structure of EIZS as a guide to systematically remold the hydrophobic active site contour in a library of 26 site-specific mutants. Remolded cyclization templates reprogram the reaction cascade not only by reproportioning products generated by the wild-type enzyme but also by generating completely new products of diverse structure. Specifically, we have tripled the overall number of characterized products generated by EIZS. Moreover, we have converted EIZS into six different sesquiterpene synthases: F96A EIZS is an (E)-beta-farnesene synthase, F96W EIZS is a zizaene synthase, F95H EIZS is a beta-curcumene synthase, F95M EIZS is a beta-acoradiene synthase, F198L EIZS is a beta-cedrene synthase, and F96V EIZS and W203F EIZS are (Z)-gamma-bisabolene synthases. Active site aromatic residues appear to be hot spots for reprogramming the cyclization cascade by manipulating the stability and conformation of critical carbocation intermediates. A majority of mutant enzymes exhibit only relatively modest 2-100-fold losses of catalytic activity, suggesting that residues responsible for triggering substrate ionization readily tolerate mutations deeper in the active site cavity.
Reprogramming the Chemodiversity of Terpenoid Cyclization by Remolding the Active Site Contour of epi-Isozizaene Synthase.,Li R, Chou WK, Himmelberger JA, Litwin KM, Harris GG, Cane DE, Christianson DW Biochemistry. 2014 Feb 25;53(7):1155-68. doi: 10.1021/bi401643u. Epub 2014 Feb, 11. PMID:24517311[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Li R, Chou WK, Himmelberger JA, Litwin KM, Harris GG, Cane DE, Christianson DW. Reprogramming the Chemodiversity of Terpenoid Cyclization by Remolding the Active Site Contour of epi-Isozizaene Synthase. Biochemistry. 2014 Feb 25;53(7):1155-68. doi: 10.1021/bi401643u. Epub 2014 Feb, 11. PMID:24517311 doi:http://dx.doi.org/10.1021/bi401643u
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