3ia5

<StructureSection load='3ia5' size='340' side='right' caption='[[3ia5]], [[Resolution|resolution]] 2.10&Aring;' scene=''>

<table><tr><td colspan='2'>[[3ia5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Jcm_11435 Jcm 11435]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IA5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IA5 FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ia4|3ia4]]</td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dyrA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=111291 JCM 11435])</td></tr>

<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ia5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ia5 OCA], [http://pdbe.org/3ia5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ia5 RCSB], [http://www.ebi.ac.uk/pdbsum/3ia5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ia5 ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/Q70YQ6_MORPR Q70YQ6_MORPR]] Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).[PIRNR:PIRNR000194]

<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ia/3ia5_consurf.spt"</scriptWhenChecked>

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== Publication Abstract from PubMed ==

We report the crystal structure of dihydrofolate reductase (DHFR) from the psychropiezophilic bacterium Moritella profunda, which was isolated from the deep ocean at 2 degrees C and 280 bar. The structure is typical of a chromosomal DHFR and we were unable to identify any obvious structural features that would suggest pressure adaptation. In particular, the core regions of the enzyme are virtually identical to those of the DHFR from the mesophile Escherichia coli. The steady-state rate at pH 9, which is limited by hydride transfer at atmospheric pressure, is roughly constant between 1 and 750 bar, falling at higher pressures. However, the value of K(M) increases with increasing pressure, and as a result k(cat)/K(M) decreases over the entire pressure range studied. Isotope effect studies showed that increasing the pressure causes a change in the rate-limiting step of the reaction. We therefore see no evidence of pressure adaptation in either the structure or the activity of this enzyme.

Are the Catalytic Properties of Enzymes from Piezophilic Organisms Pressure Adapted?,Hay S, Evans RM, Levy C, Loveridge EJ, Wang X, Leys D, Allemann RK, Scrutton NS Chembiochem. 2009 Aug 13. PMID:19681091<ref>PMID:19681091</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 3ia5" style="background-color:#fffaf0;"></div>

*[[Dihydrofolate reductase|Dihydrofolate reductase]]

[[Category: Dihydrofolate reductase]]

[[Category: Jcm 11435]]

[[Category: Allemann, R K]]

[[Category: Evans, R M]]

[[Category: Hay, S]]

[[Category: Levy, C]]

[[Category: Leys, D]]

[[Category: Loveridge, E J]]

[[Category: Scrutton, N S]]

[[Category: Wang, X]]

[[Category: Oxidoreductase]]