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| | ==Crystal structure of NfeoB from Gallionella capsiferriformans== | | ==Crystal structure of NfeoB from Gallionella capsiferriformans== |
| - | <StructureSection load='3w5i' size='340' side='right' caption='[[3w5i]], [[Resolution|resolution]] 2.15Å' scene=''> | + | <StructureSection load='3w5i' size='340' side='right'caption='[[3w5i]], [[Resolution|resolution]] 2.15Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3w5i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Galcs Galcs]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W5I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3W5I FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3w5i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallionella_capsiferriformans_ES-2 Gallionella capsiferriformans ES-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W5I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3W5I FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3w5j|3w5j]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Galf_2203 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=395494 GALCS])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3w5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w5i OCA], [https://pdbe.org/3w5i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3w5i RCSB], [https://www.ebi.ac.uk/pdbsum/3w5i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3w5i ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w5i OCA], [http://pdbe.org/3w5i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3w5i RCSB], [http://www.ebi.ac.uk/pdbsum/3w5i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3w5i ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/D9SIP4_GALCS D9SIP4_GALCS] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Galcs]] | + | [[Category: Gallionella capsiferriformans ES-2]] |
| - | [[Category: Deshpande, C N]] | + | [[Category: Large Structures]] |
| - | [[Category: Jormakka, M]] | + | [[Category: Deshpande CN]] |
| - | [[Category: McGrath, A P]] | + | [[Category: Jormakka M]] |
| - | [[Category: G protein]] | + | [[Category: McGrath AP]] |
| - | [[Category: Gtpase]]
| + | |
| - | [[Category: Iron transport]]
| + | |
| - | [[Category: Metal transport]]
| + | |
| - | [[Category: Transmembrane]]
| + | |
| Structural highlights
Function
D9SIP4_GALCS
Publication Abstract from PubMed
FeoB is a transmembrane protein involved in ferrous iron uptake in prokaryotic organisms. FeoB comprises a cytoplasmic soluble domain termed NFeoB and a C-terminal polytopic transmembrane domain. Recent structures of NFeoB have revealed two structural subdomains: a canonical GTPase domain and a five-helix helical domain. The GTPase domain hydrolyses GTP to GDP through a well characterized mechanism, a process which is required for Fe(2+) transport. In contrast, the precise role of the helical domain has not yet been fully determined. Here, the structure of the cytoplasmic domain of FeoB from Gallionella capsiferriformans is reported. Unlike recent structures of NFeoB, the G. capsiferriformans NFeoB structure is highly unusual in that it does not contain a helical domain. The crystal structures of both apo and GDP-bound protein forms a domain-swapped dimer.
Structure of an atypical FeoB G-domain reveals a putative domain-swapped dimer.,Deshpande CN, McGrath AP, Font J, Guilfoyle AP, Maher MJ, Jormakka M Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Apr 1;69(Pt 4):399-404., doi: 10.1107/S1744309113005939. Epub 2013 Mar 29. PMID:23545645[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Deshpande CN, McGrath AP, Font J, Guilfoyle AP, Maher MJ, Jormakka M. Structure of an atypical FeoB G-domain reveals a putative domain-swapped dimer. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Apr 1;69(Pt 4):399-404., doi: 10.1107/S1744309113005939. Epub 2013 Mar 29. PMID:23545645 doi:http://dx.doi.org/10.1107/S1744309113005939
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