4fbq

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==Crystal structure of a covalently fused Nbs1-Mre11 complex with two manganese ions per active site==
==Crystal structure of a covalently fused Nbs1-Mre11 complex with two manganese ions per active site==
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<StructureSection load='4fbq' size='340' side='right' caption='[[4fbq]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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<StructureSection load='4fbq' size='340' side='right'caption='[[4fbq]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4fbq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FBQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FBQ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4fbq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FBQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FBQ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fbk|4fbk]], [[4fbw|4fbw]], [[4fcx|4fcx]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fbq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fbq OCA], [http://pdbe.org/4fbq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fbq RCSB], [http://www.ebi.ac.uk/pdbsum/4fbq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fbq ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fbq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fbq OCA], [https://pdbe.org/4fbq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fbq RCSB], [https://www.ebi.ac.uk/pdbsum/4fbq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fbq ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/NBS1_SCHPO NBS1_SCHPO]] Required for DNA damage repair and S-phase DNA damage checkpoint. Involved in telomere length maintenance and maintenance of chromatin structure.<ref>PMID:12944481</ref> <ref>PMID:12944482</ref>
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[https://www.uniprot.org/uniprot/RAD32_SCHPO RAD32_SCHPO] Required for the repair of double strand breaks (DSB) caused by gamma and UV radiation. May work in conjunction with rhp51.[https://www.uniprot.org/uniprot/NBS1_SCHPO NBS1_SCHPO] Required for DNA damage repair and S-phase DNA damage checkpoint. Involved in telomere length maintenance and maintenance of chromatin structure.<ref>PMID:12944481</ref> <ref>PMID:12944482</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Mre11-Rad50-Nbs1 (MRN) complex tethers, processes and signals DNA double-strand breaks, promoting genomic stability. To understand the functional architecture of MRN, we determined the crystal structures of the Schizosaccharomyces pombe Mre11 dimeric catalytic domain alone and in complex with a fragment of Nbs1. Two Nbs1 subunits stretch around the outside of the nuclease domains of Mre11, with one subunit additionally bridging and locking the Mre11 dimer via a highly conserved asymmetrical binding motif. Our results show that Mre11 forms a flexible dimer and suggest that Nbs1 not only is a checkpoint adaptor but also functionally influences Mre11-Rad50. Clinical mutations in Mre11 are located along the Nbs1-interaction sites and weaken the Mre11-Nbs1 interaction. However, they differentially affect DNA repair and telomere maintenance in Saccharomyces cerevisiae, potentially providing insight into their different human disease pathologies.
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Structure of Mre11-Nbs1 complex yields insights into ataxia-telangiectasia-like disease mutations and DNA damage signaling.,Schiller CB, Lammens K, Guerini I, Coordes B, Feldmann H, Schlauderer F, Mockel C, Schele A, Strasser K, Jackson SP, Hopfner KP Nat Struct Mol Biol. 2012 Jun 17;19(7):693-700. doi: 10.1038/nsmb.2323. PMID:22705791<ref>PMID:22705791</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4fbq" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Fission yeast]]
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[[Category: Large Structures]]
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[[Category: Hopfner, K P]]
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[[Category: Schizosaccharomyces pombe 972h-]]
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[[Category: Lammens, K]]
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[[Category: Hopfner KP]]
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[[Category: Schiller, C B]]
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[[Category: Lammens K]]
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[[Category: Dna double-strand break repair]]
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[[Category: Schiller CB]]
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[[Category: Hydrolase]]
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[[Category: Nuclease]]
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[[Category: Protein binding]]
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Current revision

Crystal structure of a covalently fused Nbs1-Mre11 complex with two manganese ions per active site

PDB ID 4fbq

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