1mtn

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[[Image:1mtn.gif|left|200px]]
 
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{{Structure
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==BOVINE ALPHA-CHYMOTRYPSIN:BPTI CRYSTALLIZATION==
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|PDB= 1mtn |SIZE=350|CAPTION= <scene name='initialview01'>1mtn</scene>, resolution 2.8&Aring;
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<StructureSection load='1mtn' size='340' side='right'caption='[[1mtn]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1mtn]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MTN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MTN FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mtn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mtn OCA], [https://pdbe.org/1mtn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mtn RCSB], [https://www.ebi.ac.uk/pdbsum/1mtn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mtn ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mtn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mtn OCA], [http://www.ebi.ac.uk/pdbsum/1mtn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mtn RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/CTRA_BOVIN CTRA_BOVIN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mt/1mtn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mtn ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of bovine alpha-chymotrypsin (alpha-CHT) in complex with the bovine basic pancreatic trypsin inhibitor (BPTI) has been solved and refined at 2.8 A resolution (R-factor = 0.18). The proteinase:inhibitor complex forms a compact dimer (two alpha-CHT and two BPTI molecules), which may be stabilized by surface-bound sulphate ions, in the crystalline state. Each BPTI molecule, at opposite ends, is contacting both proteinase molecules in the dimer, through the reactive site loop and through residues next to the inhibitor's C-terminal region. Specific recognition between alpha-CHT and BPTI occurs at the (re)active site interface according to structural rules inferred from the analysis of homologous serine proteinase:inhibitor complexes. Lys15, the P1 residue of BPTI, however, does not occupy the alpha-CHT S1 specificity pocket, being hydrogen bonded to backbone atoms of the enzyme surface residues Gly216 and Ser217.
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'''BOVINE ALPHA-CHYMOTRYPSIN:BPTI CRYSTALLIZATION'''
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Crystal structure of the bovine alpha-chymotrypsin:Kunitz inhibitor complex. An example of multiple protein:protein recognition sites.,Capasso C, Rizzi M, Menegatti E, Ascenzi P, Bolognesi M J Mol Recognit. 1997 Jan-Feb;10(1):26-35. PMID:9179777<ref>PMID:9179777</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1mtn" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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The crystal structure of bovine alpha-chymotrypsin (alpha-CHT) in complex with the bovine basic pancreatic trypsin inhibitor (BPTI) has been solved and refined at 2.8 A resolution (R-factor = 0.18). The proteinase:inhibitor complex forms a compact dimer (two alpha-CHT and two BPTI molecules), which may be stabilized by surface-bound sulphate ions, in the crystalline state. Each BPTI molecule, at opposite ends, is contacting both proteinase molecules in the dimer, through the reactive site loop and through residues next to the inhibitor's C-terminal region. Specific recognition between alpha-CHT and BPTI occurs at the (re)active site interface according to structural rules inferred from the analysis of homologous serine proteinase:inhibitor complexes. Lys15, the P1 residue of BPTI, however, does not occupy the alpha-CHT S1 specificity pocket, being hydrogen bonded to backbone atoms of the enzyme surface residues Gly216 and Ser217.
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*[[BPTI 3D structures|BPTI 3D structures]]
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*[[Chymotrypsin 3D structures|Chymotrypsin 3D structures]]
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==About this Structure==
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== References ==
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1MTN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MTN OCA].
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<references/>
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of the bovine alpha-chymotrypsin:Kunitz inhibitor complex. An example of multiple protein:protein recognition sites., Capasso C, Rizzi M, Menegatti E, Ascenzi P, Bolognesi M, J Mol Recognit. 1997 Jan-Feb;10(1):26-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9179777 9179777]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Chymotrypsin]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Ascenzi P]]
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[[Category: Ascenzi, P.]]
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[[Category: Bolognesi M]]
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[[Category: Bolognesi, M.]]
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[[Category: Capasso C]]
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[[Category: Capasso, C.]]
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[[Category: Menegatti E]]
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[[Category: Menegatti, E.]]
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[[Category: Rizzi M]]
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[[Category: Rizzi, M.]]
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[[Category: complex]]
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[[Category: hydrolase]]
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[[Category: protease inhibitor]]
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[[Category: serine]]
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[[Category: trypsin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:20:57 2008''
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Current revision

BOVINE ALPHA-CHYMOTRYPSIN:BPTI CRYSTALLIZATION

PDB ID 1mtn

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