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| | ==Human enterovirus 71 3C protease== | | ==Human enterovirus 71 3C protease== |
| - | <StructureSection load='3osy' size='340' side='right' caption='[[3osy]], [[Resolution|resolution]] 2.99Å' scene=''> | + | <StructureSection load='3osy' size='340' side='right'caption='[[3osy]], [[Resolution|resolution]] 2.99Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3osy]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Ev-71 Ev-71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OSY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OSY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3osy]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterovirus_A71 Enterovirus A71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OSY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OSY FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">3C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39054 EV-71])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9923Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3osy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3osy OCA], [http://pdbe.org/3osy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3osy RCSB], [http://www.ebi.ac.uk/pdbsum/3osy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3osy ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3osy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3osy OCA], [https://pdbe.org/3osy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3osy RCSB], [https://www.ebi.ac.uk/pdbsum/3osy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3osy ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/B8YLV9_HE71 B8YLV9_HE71] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[3C protease|3C protease]] | + | *[[Virus protease 3D structures|Virus protease 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ev-71]] | + | [[Category: Enterovirus A71]] |
| - | [[Category: Cui, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Fan, T]] | + | [[Category: Cui S]] |
| - | [[Category: Wang, J]] | + | [[Category: Fan T]] |
| - | [[Category: Wang, M]] | + | [[Category: Wang J]] |
| - | [[Category: Beta-ribbon]]
| + | [[Category: Wang M]] |
| - | [[Category: Chymotrypsin fold]]
| + | |
| - | [[Category: Cstf-64]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Mav]]
| + | |
| - | [[Category: Nucleus]]
| + | |
| - | [[Category: Protease]]
| + | |
| - | [[Category: Rig-1]]
| + | |
| - | [[Category: Six-beta barrel]]
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| Structural highlights
Function
B8YLV9_HE71
Publication Abstract from PubMed
Human enterovirus 71 (EV71) is the major pathogen that causes hand, foot and mouth disease that particularly affects young children. Growing hand, foot and mouth disease outbreaks were observed worldwide in recent years and caused devastating losses both economically and politically. However, vaccines or effective drugs are unavailable to date. The genome of EV71 consists of a positive sense, single-stranded RNA of approximately 7400 bp, encoding a large precursor polyprotein that requires proteolytic processing to generate mature viral proteins. The proteolytic processing mainly depends on EV71 3C protease (3C(pro)) that possesses both proteolysis and RNA binding activities, which enable the protease to perform multiple tasks in viral replication and pathogen-host interactions. The central roles played by EV71 3C(pro) make it an appealing target for antiviral drug development. We determined the first crystal structure of EV71 3C(pro) and analyzed its enzymatic activity. The crystal structure shows that EV71 3C(pro) has a typical chymotrypsin-like fold that is common in picornaviral 3C(pro). Strikingly, we found an important surface loop, also denoted as beta-ribbon, which adopts a novel open conformation in EV71 3C(pro). We identified two important residues located at the base of the beta-ribbon, Gly123 and His133, which form hinges that govern the intrinsic flexibility of the ribbon. Structure-guided mutagenesis studies revealed that the hinge residues are important to EV71 3C(pro) proteolytic activities. In summary, our work provides the first structural insight into EV71 3C(pro), including a mobile beta-ribbon, which is relevant to the proteolytic mechanism. Our data also provides a framework for structure-guided inhibitor design against EV71 3C(pro).
Crystal Structure of Human Enterovirus 71 3C Protease.,Cui S, Wang J, Fan T, Qin B, Guo L, Lei X, Wang J, Wang M, Jin Q J Mol Biol. 2011 May 6;408(3):449-61. Epub 2011 Mar 17. PMID:21396941[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Cui S, Wang J, Fan T, Qin B, Guo L, Lei X, Wang J, Wang M, Jin Q. Crystal Structure of Human Enterovirus 71 3C Protease. J Mol Biol. 2011 May 6;408(3):449-61. Epub 2011 Mar 17. PMID:21396941 doi:10.1016/j.jmb.2011.03.007
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