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| | ==Structure of a CENP-A-Histone H4 Heterodimer in complex with chaperone HJURP== | | ==Structure of a CENP-A-Histone H4 Heterodimer in complex with chaperone HJURP== |
| - | <StructureSection load='3r45' size='340' side='right' caption='[[3r45]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='3r45' size='340' side='right'caption='[[3r45]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3r45]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R45 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R45 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3r45]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R45 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R45 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CENPA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), HJURP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r45 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r45 OCA], [http://pdbe.org/3r45 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3r45 RCSB], [http://www.ebi.ac.uk/pdbsum/3r45 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3r45 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r45 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r45 OCA], [https://pdbe.org/3r45 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r45 RCSB], [https://www.ebi.ac.uk/pdbsum/3r45 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r45 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CENPA_HUMAN CENPA_HUMAN]] Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. The CENPA-H4 heterotetramer can bind DNA by itself (in vitro).<ref>PMID:20739937</ref> <ref>PMID:21478274</ref> [[http://www.uniprot.org/uniprot/HJURP_HUMAN HJURP_HUMAN]] Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.<ref>PMID:19410544</ref> <ref>PMID:19410545</ref> | + | [https://www.uniprot.org/uniprot/HJURP_HUMAN HJURP_HUMAN] Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.<ref>PMID:19410544</ref> <ref>PMID:19410545</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Centromere protein|Centromere protein]] | + | *[[Centromere protein 3D structure|Centromere protein 3D structure]] |
| - | *[[Histone|Histone]] | + | *[[Histone 3D structures|Histone 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Fang, J]] | + | [[Category: Large Structures]] |
| - | [[Category: Hu, H]] | + | [[Category: Fang J]] |
| - | [[Category: Huang, H]] | + | [[Category: Hu H]] |
| - | [[Category: Li, G]] | + | [[Category: Huang H]] |
| - | [[Category: Li, Y]] | + | [[Category: Li G]] |
| - | [[Category: Liu, Y]] | + | [[Category: Li Y]] |
| - | [[Category: Shi, Y]] | + | [[Category: Liu Y]] |
| - | [[Category: Wang, J]] | + | [[Category: Shi Y]] |
| - | [[Category: Wang, M]] | + | [[Category: Wang J]] |
| - | [[Category: Xu, R M]] | + | [[Category: Wang M]] |
| - | [[Category: Yang, N]] | + | [[Category: Xu RM]] |
| - | [[Category: Yao, X]] | + | [[Category: Yang N]] |
| - | [[Category: Cenp-a]]
| + | [[Category: Yao X]] |
| - | [[Category: Centromere]]
| + | |
| - | [[Category: Histone chaperone]]
| + | |
| - | [[Category: Histone fold]]
| + | |
| - | [[Category: Hjurp]]
| + | |
| - | [[Category: Nuclear protein]]
| + | |
| Structural highlights
Function
HJURP_HUMAN Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.[1] [2]
Publication Abstract from PubMed
In higher eukaryotes, the centromere is epigenetically specified by the histone H3 variant Centromere Protein-A (CENP-A). Deposition of CENP-A to the centromere requires histone chaperone HJURP (Holliday junction recognition protein). The crystal structure of an HJURP-CENP-A-histone H4 complex shows that HJURP binds a CENP-A-H4 heterodimer. The C-terminal beta-sheet domain of HJURP caps the DNA-binding region of the histone heterodimer, preventing it from spontaneous association with DNA. Our analysis also revealed a novel site in CENP-A that distinguishes it from histone H3 in its ability to bind HJURP. These findings provide key information for specific recognition of CENP-A and mechanistic insights into the process of centromeric chromatin assembly.
Structure of a CENP-A-histone H4 heterodimer in complex with chaperone HJURP.,Hu H, Liu Y, Wang M, Fang J, Huang H, Yang N, Li Y, Wang J, Yao X, Shi Y, Li G, Xu RM Genes Dev. 2011 May 1;25(9):901-6. Epub 2011 Apr 8. PMID:21478274[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Foltz DR, Jansen LE, Bailey AO, Yates JR 3rd, Bassett EA, Wood S, Black BE, Cleveland DW. Centromere-specific assembly of CENP-a nucleosomes is mediated by HJURP. Cell. 2009 May 1;137(3):472-84. PMID:19410544 doi:http://dx.doi.org/S0092-8674(09)00253-0
- ↑ Dunleavy EM, Roche D, Tagami H, Lacoste N, Ray-Gallet D, Nakamura Y, Daigo Y, Nakatani Y, Almouzni-Pettinotti G. HJURP is a cell-cycle-dependent maintenance and deposition factor of CENP-A at centromeres. Cell. 2009 May 1;137(3):485-97. PMID:19410545 doi:http://dx.doi.org/S0092-8674(09)00254-2
- ↑ Hu H, Liu Y, Wang M, Fang J, Huang H, Yang N, Li Y, Wang J, Yao X, Shi Y, Li G, Xu RM. Structure of a CENP-A-histone H4 heterodimer in complex with chaperone HJURP. Genes Dev. 2011 May 1;25(9):901-6. Epub 2011 Apr 8. PMID:21478274 doi:10.1101/gad.2045111
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