3zbj

From Proteopedia

(Difference between revisions)

Current revision

Fitting results in the I-layer of the subnanometer structure of the bacterial pKM101 type IV secretion system core complex digested with elastase

3zbj, resolution 8.50Å

Structural highlights

3zbj is a 14 chain structure with sequence from Escherichia coli BL21. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 8.5Å
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q46704_ECOLX

Publication Abstract from PubMed

Type IV secretion (T4S) systems are able to transport DNAs and/or proteins through the membranes of bacteria. They form large multiprotein complexes consisting of 12 proteins termed VirB1-11 and VirD4. VirB7, 9 and 10 assemble into a 1.07 MegaDalton membrane-spanning core complex (CC), around which all other components assemble. This complex is made of two parts, the O-layer inserted in the outer membrane and the I-layer inserted in the inner membrane. While the structure of the O-layer has been solved by X-ray crystallography, there is no detailed structural information on the I-layer. Using high-resolution cryo-electron microscopy and molecular modelling combined with biochemical approaches, we determined the I-layer structure and located its various components in the electron density. Our results provide new structural insights on the CC, from which the essential features of T4S system mechanisms can be derived.

Structure of a bacterial type IV secretion core complex at subnanometre resolution.,Rivera-Calzada A, Fronzes R, Savva CG, Chandran V, Lian PW, Laeremans T, Pardon E, Steyaert J, Remaut H, Waksman G, Orlova EV EMBO J. 2013 Mar 19. doi: 10.1038/emboj.2013.58. PMID:23511972^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rivera-Calzada A, Fronzes R, Savva CG, Chandran V, Lian PW, Laeremans T, Pardon E, Steyaert J, Remaut H, Waksman G, Orlova EV. Structure of a bacterial type IV secretion core complex at subnanometre resolution. EMBO J. 2013 Mar 19. doi: 10.1038/emboj.2013.58. PMID:23511972 doi:http://dx.doi.org/10.1038/emboj.2013.58

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3zbj"

@@ Line 1: / Line 1: @@
 ==Fitting results in the I-layer of the subnanometer structure of the bacterial pKM101 type IV secretion system core complex digested with elastase==
-<StructureSection load='3zbj' size='340' side='right' caption='[[3zbj]], [[Resolution|resolution]] 8.50&Aring;' scene=''>
+<SX load='3zbj' size='340' side='right' viewer='molstar' caption='[[3zbj]], [[Resolution|resolution]] 8.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3zbj]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecobb Ecobb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZBJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZBJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3zbj]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21 Escherichia coli BL21]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZBJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZBJ FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zbi|3zbi]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8.5&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zbj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zbj OCA], [http://pdbe.org/3zbj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zbj RCSB], [http://www.ebi.ac.uk/pdbsum/3zbj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zbj ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zbj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zbj OCA], [https://pdbe.org/3zbj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zbj RCSB], [https://www.ebi.ac.uk/pdbsum/3zbj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zbj ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q46704_ECOLX Q46704_ECOLX]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 21: @@
 <references/>
 __TOC__
-</StructureSection>
+</SX>
-[[Category: Ecobb]]
+[[Category: Escherichia coli BL21]]
-[[Category: Chandran, V]]
+[[Category: Large Structures]]
-[[Category: Fronzes, R]]
+[[Category: Chandran V]]
-[[Category: Laeremans, T]]
+[[Category: Fronzes R]]
-[[Category: Lian, P W]]
+[[Category: Laeremans T]]
-[[Category: Orlova, E V]]
+[[Category: Lian PW]]
-[[Category: Pardon, E]]
+[[Category: Orlova EV]]
-[[Category: Remaut, H]]
+[[Category: Pardon E]]
-[[Category: Rivera-Calzada, A]]
+[[Category: Remaut H]]
-[[Category: Savva, C G]]
+[[Category: Rivera-Calzada A]]
-[[Category: Steyaert, J]]
+[[Category: Savva CG]]
-[[Category: Waksman, G]]
+[[Category: Steyaert J]]
-[[Category: Bacterial secretion]]
+[[Category: Waksman G]]
-[[Category: Cell adhesion]]

3zbj

From Proteopedia

Current revision

Fitting results in the I-layer of the subnanometer structure of the bacterial pKM101 type IV secretion system core complex digested with elastase

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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