2xd3

From Proteopedia

(Difference between revisions)

Current revision

The crystal structure of MalX from Streptococcus pneumoniae in complex with maltopentaose.

Structural highlights

2xd3 is a 1 chain structure with sequence from Streptococcus pneumoniae TIGR4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MALX_STRPN Binding protein for maltose and maltodextrin; involved in its cellular uptake.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The genome of Streptococcus pneumoniae strains, as typified by the TIGR4 strain, contain several genes encoding proteins putatively involved in alpha-glucan degradation, modification and synthesis. The extracellular components comprise an ATP binding cassette-transporter with its solute binding protein, MalX, and the hydrolytic enzyme SpuA. We show that of the commonly occurring exogenous alpha-glucans, S. pneumoniae TIGR4 is only able to grow on glycogen in a MalX- and SpuA-dependent manner. SpuA is able to degrade glycogen into a ladder of alpha-1,4-glucooligosaccharides while the high-affinity interaction (K(a) approximately 10(6) M(-1)) of MalX with maltooligosaccharides plays a key role in promoting the selective uptake of the glycogen degradation products that are produced by SpuA. The X-ray crystallographic analyses of apo- and complexed MalX illuminate the protein's specificity for the degradation products of glycogen and its striking ability to recognize the helical structure of the ligand. Overall, the results of this work provide new structural and functional insight into streptococcal alpha-glucan metabolism while supplying biochemical support for the hypothesis that the substrate of the S. pneumoniaealpha-glucan metabolizing machinery is glycogen, which in a human host is abundant in lung epithelial cells, a common target for invasive S. pneumoniae.

The molecular basis of glycogen breakdown and transport in Streptococcus pneumoniae.,Abbott DW, Higgins MA, Hyrnuik S, Pluvinage B, Lammerts van Bueren A, Boraston AB Mol Microbiol. 2010 Jul 1;77(1):183-99. Epub 2010 May 19. PMID:20497336^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Abbott DW, Higgins MA, Hyrnuik S, Pluvinage B, Lammerts van Bueren A, Boraston AB. The molecular basis of glycogen breakdown and transport in Streptococcus pneumoniae. Mol Microbiol. 2010 Jul 1;77(1):183-99. Epub 2010 May 19. PMID:20497336 doi:10.1111/j.1365-2958.2010.07199.x

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xd3"

@@ Line 1: / Line 1: @@
-==THE CRYSTAL STRUCTURE OF MALX FROM STREPTOCOCCUS PNEUMONIAE IN COMPLEX WITH MALTOPENTAOSE.==
+==The crystal structure of MalX from Streptococcus pneumoniae in complex with maltopentaose.==
-<StructureSection load='2xd3' size='340' side='right' caption='[[2xd3]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+<StructureSection load='2xd3' size='340' side='right'caption='[[2xd3]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2xd3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strpn Strpn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XD3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XD3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2xd3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XD3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XD3 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xd2|2xd2]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xd3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xd3 OCA], [http://pdbe.org/2xd3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xd3 RCSB], [http://www.ebi.ac.uk/pdbsum/2xd3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xd3 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xd3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xd3 OCA], [https://pdbe.org/2xd3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xd3 RCSB], [https://www.ebi.ac.uk/pdbsum/2xd3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xd3 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/MALX_STRPN MALX_STRPN]] Binding protein for maltose and maltodextrin; involved in its cellular uptake.
+[https://www.uniprot.org/uniprot/MALX_STRPN MALX_STRPN] Binding protein for maltose and maltodextrin; involved in its cellular uptake.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xd/2xd3_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xd/2xd3_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 31: / Line 31: @@
 ==See Also==
-*[[Maltose-binding protein|Maltose-binding protein]]
+*[[Maltose-binding protein 3D structures|Maltose-binding protein 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Strpn]]
+[[Category: Large Structures]]
-[[Category: Abbott, D W]]
+[[Category: Streptococcus pneumoniae TIGR4]]
-[[Category: Boraston, A B]]
+[[Category: Abbott DW]]
-[[Category: Bueren, A Lammerts Van]]
+[[Category: Boraston AB]]
-[[Category: Higgins, M A]]
+[[Category: Higgins MA]]
-[[Category: Hyrnuik, S]]
+[[Category: Hyrnuik S]]
-[[Category: Pluvinage, B]]
+[[Category: Lammerts van Bueren A]]
-[[Category: Alpha-glucan]]
+[[Category: Pluvinage B]]
-[[Category: Membrane protein]]
-[[Category: Solute-binding protein]]
-[[Category: Sugar binding protein]]
-[[Category: Sugar transport]]
-[[Category: Virulence]]

2xd3

From Proteopedia

Current revision

The crystal structure of MalX from Streptococcus pneumoniae in complex with maltopentaose.

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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