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| | ==Cpn0482 : the arginine binding protein from the periplasm of chlamydia Pneumoniae== | | ==Cpn0482 : the arginine binding protein from the periplasm of chlamydia Pneumoniae== |
| - | <StructureSection load='3n26' size='340' side='right' caption='[[3n26]], [[Resolution|resolution]] 2.10Å' scene=''> | + | <StructureSection load='3n26' size='340' side='right'caption='[[3n26]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3n26]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_vr-2282 Atcc vr-2282]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3g41 3g41]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N26 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N26 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3n26]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydia_pneumoniae Chlamydia pneumoniae]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3g41 3g41]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N26 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N26 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1hsl|1hsl]], [[3del|3del]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">artJ, CP_0272 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83558 ATCC VR-2282])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n26 OCA], [https://pdbe.org/3n26 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n26 RCSB], [https://www.ebi.ac.uk/pdbsum/3n26 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n26 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n26 OCA], [http://pdbe.org/3n26 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3n26 RCSB], [http://www.ebi.ac.uk/pdbsum/3n26 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3n26 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ARTJ_CHLPN ARTJ_CHLPN]] Probably part of an ABC transporter complex involved in arginine transport. Binds arginine. Interacts with host epithelial cells, suggesting a role in host-cell adhesion during infection.<ref>PMID:20592031</ref> | + | [https://www.uniprot.org/uniprot/ARTJ_CHLPN ARTJ_CHLPN] Probably part of an ABC transporter complex involved in arginine transport. Binds arginine. Interacts with host epithelial cells, suggesting a role in host-cell adhesion during infection.<ref>PMID:20592031</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> | | Check<jmol> |
| | <jmolCheckbox> | | <jmolCheckbox> |
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n2/3n26_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n2/3n26_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
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| | ==See Also== | | ==See Also== |
| - | *[[ABC transporter|ABC transporter]] | + | *[[ABC transporter 3D structures|ABC transporter 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc vr-2282]] | + | [[Category: Chlamydia pneumoniae]] |
| - | [[Category: Garcia, C]] | + | [[Category: Large Structures]] |
| - | [[Category: Grandi, G]] | + | [[Category: Garcia C]] |
| - | [[Category: Structural genomic]] | + | [[Category: Grandi G]] |
| - | [[Category: Petit, P]] | + | [[Category: Petit P]] |
| - | [[Category: Soriani, M]] | + | [[Category: Soriani M]] |
| - | [[Category: Vuillard, L]] | + | [[Category: Vuillard L]] |
| - | [[Category: Bacabs - eu fp6 programme]]
| + | |
| - | [[Category: Msgp]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| - | [[Category: Vaccine development]]
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| Structural highlights
Function
ARTJ_CHLPN Probably part of an ABC transporter complex involved in arginine transport. Binds arginine. Interacts with host epithelial cells, suggesting a role in host-cell adhesion during infection.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
We present an interdisciplinary approach that, by incorporating a range of experimental and computational techniques, allows the identification and characterization of functional/immunogenic domains. This approach has been applied to ArtJ, an arginine binding protein whose orthologs in Chlamydiae trachomatis (CT ArtJ) and pneumoniae (CPn ArtJ) are shown to have different immunogenic properties despite a high sequence similarity (60% identity). We have solved the crystallographic structures of CT ArtJ and CPn ArtJ, which are found to display a type-II transporter fold organized in two alpha-beta domains with the arginine-binding region at their interface. Although ArtJ is considered to belong to the periplasm, we found that both domains contain regions exposed on the bacterial surface. Moreover, we show that recombinant ArtJ binds to epithelial cells in vitro, suggesting a role for ArtJ in host-cell adhesion during Chlamydia infection. Experimental epitope mapping and computational analysis of physico-chemical determinants of antibody recognition revealed that immunogenic epitopes reside mainly in the terminal (D1) domain of both CPn and CT ArtJ, while the surface properties of the respective binding-prone regions appear sufficiently different to assume divergent immunogenic behavior. Neutralization assays revealed that sera raised against CPn ArtJ D1 partially reduce both CPn and CT infectivity in vitro, suggesting that functional antibodies directed against this domain may potentially impair chlamydial infectivity. These findings suggest that the approach presented here, combining functional and structure-based analyses of evolutionary-related antigens can be a valuable tool for the identification of cross-species immunogenic epitopes for vaccine development.
Exploiting antigenic diversity for vaccine design: the Chlamydia ArtJ paradigm.,Soriani M, Petit P, Grifantini R, Petracca R, Gancitano G, Frigimelica E, Nardelli F, Garcia C, Spinelli S, Scarabelli G, Fiorucci S, Affentranger R, Ferrer-Navarro M, Zacharias M, Colombo G, Vuillard L, Daura X, Grandi G J Biol Chem. 2010 Jun 30. PMID:20592031[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Soriani M, Petit P, Grifantini R, Petracca R, Gancitano G, Frigimelica E, Nardelli F, Garcia C, Spinelli S, Scarabelli G, Fiorucci S, Affentranger R, Ferrer-Navarro M, Zacharias M, Colombo G, Vuillard L, Daura X, Grandi G. Exploiting antigenic diversity for vaccine design: the Chlamydia ArtJ paradigm. J Biol Chem. 2010 Jun 30. PMID:20592031 doi:10.1074/jbc.M110.118513
- ↑ Soriani M, Petit P, Grifantini R, Petracca R, Gancitano G, Frigimelica E, Nardelli F, Garcia C, Spinelli S, Scarabelli G, Fiorucci S, Affentranger R, Ferrer-Navarro M, Zacharias M, Colombo G, Vuillard L, Daura X, Grandi G. Exploiting antigenic diversity for vaccine design: the Chlamydia ArtJ paradigm. J Biol Chem. 2010 Jun 30. PMID:20592031 doi:10.1074/jbc.M110.118513
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