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| | ==Crystal structure of engineered Naja Nigricollis toxin alpha== | | ==Crystal structure of engineered Naja Nigricollis toxin alpha== |
| - | <StructureSection load='3nds' size='340' side='right' caption='[[3nds]], [[Resolution|resolution]] 1.20Å' scene=''> | + | <StructureSection load='3nds' size='340' side='right'caption='[[3nds]], [[Resolution|resolution]] 1.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3nds]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NDS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NDS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3nds]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Naja_pallida Naja pallida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NDS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NDS FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1iq9|1iq9]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nds OCA], [http://pdbe.org/3nds PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nds RCSB], [http://www.ebi.ac.uk/pdbsum/3nds PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nds ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nds OCA], [https://pdbe.org/3nds PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nds RCSB], [https://www.ebi.ac.uk/pdbsum/3nds PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nds ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NXS1_NAJPA NXS1_NAJPA]] Binds with high affinity to muscular nicotinic acetylcholine receptors (nAChRs), and with low affinity to neuronal alpha-7 nAChRs. Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site.<ref>PMID:9305882</ref> | + | [https://www.uniprot.org/uniprot/3S11_NAJPA 3S11_NAJPA] Binds with high affinity to muscular nicotinic acetylcholine receptors (nAChRs) (tested on Torpedo marmorata AChR, Kd=0.07 nM) and with low affinity to neuronal alpha-7/CHRNA7 nAChRs (tested on chimeric receptor, Kd=3 uM) and inhibit acetylcholine from binding to the receptor, thereby impairing neuromuscular transmission (PubMed:9305882). Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site.<ref>PMID:9305882</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> | | Check<jmol> |
| | <jmolCheckbox> | | <jmolCheckbox> |
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nd/3nds_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nd/3nds_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drevet, P]] | + | [[Category: Large Structures]] |
| - | [[Category: Gallopin, M]] | + | [[Category: Naja pallida]] |
| - | [[Category: Gregory, G]] | + | [[Category: Drevet P]] |
| - | [[Category: Stura, E A]] | + | [[Category: Gallopin M]] |
| - | [[Category: Vandame, M]]
| + | [[Category: Gregory G]] |
| - | [[Category: Disulfide pairing]] | + | [[Category: Stura EA]] |
| - | [[Category: Folding intermediate]] | + | [[Category: Vandame M]] |
| - | [[Category: Snake toxin]] | + | |
| - | [[Category: Three finger toxin fold]]
| + | |
| - | [[Category: Toxin]]
| + | |
| - | [[Category: Venom]]
| + | |
| Structural highlights
Function
3S11_NAJPA Binds with high affinity to muscular nicotinic acetylcholine receptors (nAChRs) (tested on Torpedo marmorata AChR, Kd=0.07 nM) and with low affinity to neuronal alpha-7/CHRNA7 nAChRs (tested on chimeric receptor, Kd=3 uM) and inhibit acetylcholine from binding to the receptor, thereby impairing neuromuscular transmission (PubMed:9305882). Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Much has been learned about the folding of proteins from comparative studies of the folding of proteins that are related in sequence and structure. Observation of the effects of mutations helps account for sequence-specific properties and large variations in folding rates observed in homologous proteins, which are not explained by structure-derived descriptions. The folding kinetics of variants of a beta-stranded protein, toxin alpha from Naja nigricollis, depends on the length of their loop lk1. These proteins, named Tox60, Tox61, and Tox62, contain four disulfide bonds. We show that their oxidative refolding pathways are similar. Differences in these pathways are restricted to the last step of the reaction, that is, the closure of the last disulfide. At this step, two species of three-disulfide intermediates are observed: intermediate C lacking the B3 disulfide and intermediate D lacking the B2 disulfide. Surprisingly, D is the most productive intermediate for Tox61 despite the low accessibility of its free cysteines. However, in the case of Tox62, its conversion efficiency drops by 2 orders of magnitude and C becomes the most productive intermediate. NMR was used in order to study the structural dynamics of each of these intermediates. Both three-disulfide intermediates of Tox61 exist in two forms, exchanging on the 1- to 100-ms scale. One of these forms is structurally very close to the native Tox61, whereas the other is always significantly more flexible on a picosecond-to-nanosecond timescale. On the other hand, in the case of Tox62, the three-disulfide intermediates only show a native-like structure. The higher conformational heterogeneity of Tox61 intermediate D allows an increased accessibility of its free cysteines to oxidative agents, which explains its faster native disulfide formation. Thus, residue deletion in loop lk1 probably abrogates stabilizing intramolecular interactions, creates conformational heterogeneity, and increases the folding rate of Tox60 and Tox61 compared to Tox62.
Conformational exchange is critical for the productivity of an oxidative folding intermediate with buried free cysteines.,Gross G, Gallopin M, Vandame M, Couprie J, Stura E, Zinn-Justin S, Drevet P J Mol Biol. 2010 Oct 22;403(2):299-312. Epub 2010 Sep 8. PMID:20804768[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Servent D, Winckler-Dietrich V, Hu HY, Kessler P, Drevet P, Bertrand D, Menez A. Only snake curaremimetic toxins with a fifth disulfide bond have high affinity for the neuronal alpha7 nicotinic receptor. J Biol Chem. 1997 Sep 26;272(39):24279-86. PMID:9305882
- ↑ Gross G, Gallopin M, Vandame M, Couprie J, Stura E, Zinn-Justin S, Drevet P. Conformational exchange is critical for the productivity of an oxidative folding intermediate with buried free cysteines. J Mol Biol. 2010 Oct 22;403(2):299-312. Epub 2010 Sep 8. PMID:20804768 doi:10.1016/j.jmb.2010.07.048
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