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| | ==Crystal structure of the leptospiral antigen Lp49== | | ==Crystal structure of the leptospiral antigen Lp49== |
| - | <StructureSection load='3bws' size='340' side='right' caption='[[3bws]], [[Resolution|resolution]] 1.99Å' scene=''> | + | <StructureSection load='3bws' size='340' side='right'caption='[[3bws]], [[Resolution|resolution]] 1.99Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3bws]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"leptospira_icteroides"_noguchi_1919 "leptospira icteroides" noguchi 1919]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BWS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3bws]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptospira_interrogans Leptospira interrogans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BWS FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.99Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LIC10793 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=173 "Leptospira icteroides" Noguchi 1919])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bws OCA], [https://pdbe.org/3bws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bws RCSB], [https://www.ebi.ac.uk/pdbsum/3bws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bws ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bws OCA], [http://pdbe.org/3bws PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bws RCSB], [http://www.ebi.ac.uk/pdbsum/3bws PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bws ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/LP49_LEPIC LP49_LEPIC] May be involved in virulence. Binds human plasminogen (PLG) and stimulates its proteolytic cleavage to enzymatically active plasmin in the presence of an urokinase-type PLG activator in vitro. Activated plasmin has proteolytic activity which may help the bacteria to spread throughout the host by degrading extracellular matrix components, facilitating tissue penetration and invasion.<ref>PMID:20582320</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> | | Check<jmol> |
| | <jmolCheckbox> | | <jmolCheckbox> |
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/3bws_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/3bws_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Leptospira icteroides noguchi 1919]] | + | [[Category: Large Structures]] |
| - | [[Category: Giuseppe, P O]] | + | [[Category: Leptospira interrogans]] |
| - | [[Category: Guimaraes, B G]] | + | [[Category: Giuseppe PO]] |
| - | [[Category: Nascimento, A L.T O]] | + | [[Category: Guimaraes BG]] |
| - | [[Category: Neves, F O]] | + | [[Category: Nascimento ALTO]] |
| - | [[Category: 7-bladed beta propeller]] | + | [[Category: Neves FO]] |
| - | [[Category: Immunoglobulin-like]]
| + | |
| - | [[Category: Two-domain]]
| + | |
| - | [[Category: Unknown function]]
| + | |
| Structural highlights
Function
LP49_LEPIC May be involved in virulence. Binds human plasminogen (PLG) and stimulates its proteolytic cleavage to enzymatically active plasmin in the presence of an urokinase-type PLG activator in vitro. Activated plasmin has proteolytic activity which may help the bacteria to spread throughout the host by degrading extracellular matrix components, facilitating tissue penetration and invasion.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Pathogenic Leptospira is the etiological agent of leptospirosis, a life-threatening disease that affects populations worldwide. Currently available vaccines have limited effectiveness and therapeutic interventions are complicated by the difficulty in making an early diagnosis of leptospirosis. The genome of Leptospira interrogans was recently sequenced and comparative genomic analysis contributed to the identification of surface antigens, potential candidates for development of new vaccines and serodiagnosis. Lp49 is a membrane-associated protein recognized by antibodies present in sera from early and convalescent phases of leptospirosis patients. Its crystal structure was determined by single-wavelength anomalous diffraction using selenomethionine-labelled crystals and refined at 2.0 A resolution. Lp49 is composed of two domains and belongs to the all-beta-proteins class. The N-terminal domain folds in an immunoglobulin-like beta-sandwich structure, whereas the C-terminal domain presents a seven-bladed beta-propeller fold. Structural analysis of Lp49 indicates putative protein-protein binding sites, suggesting a role in Leptospira-host interaction. This is the first crystal structure of a leptospiral antigen described to date.
The leptospiral antigen Lp49 is a two-domain protein with putative protein binding function.,Giuseppe PO, Neves FO, Nascimento AL, Guimaraes BG J Struct Biol. 2008 Jul;163(1):53-60. Epub 2008 Apr 14. PMID:18508281[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Vieira ML, Atzingen MV, Oliveira TR, Oliveira R, Andrade DM, Vasconcellos SA, Nascimento AL. In vitro identification of novel plasminogen-binding receptors of the pathogen Leptospira interrogans. PLoS One. 2010 Jun 22;5(6):e11259. PMID:20582320 doi:10.1371/journal.pone.0011259
- ↑ Giuseppe PO, Neves FO, Nascimento AL, Guimaraes BG. The leptospiral antigen Lp49 is a two-domain protein with putative protein binding function. J Struct Biol. 2008 Jul;163(1):53-60. Epub 2008 Apr 14. PMID:18508281 doi:10.1016/j.jsb.2008.04.003
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