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| - | [[Image:1myk.gif|left|200px]] | |
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| - | {{Structure
| + | ==CRYSTAL STRUCTURE, FOLDING, AND OPERATOR BINDING OF THE HYPERSTABLE ARC REPRESSOR MUTANT PL8== |
| - | |PDB= 1myk |SIZE=350|CAPTION= <scene name='initialview01'>1myk</scene>, resolution 2.4Å
| + | <StructureSection load='1myk' size='340' side='right'caption='[[1myk]], [[Resolution|resolution]] 2.40Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND=
| + | <table><tr><td colspan='2'>[[1myk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_virus_P22 Salmonella virus P22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MYK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MYK FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | |GENE= MUTATED ARC GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10754 Enterobacteria phage P22])
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1myk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1myk OCA], [https://pdbe.org/1myk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1myk RCSB], [https://www.ebi.ac.uk/pdbsum/1myk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1myk ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | == Function == |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1myk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1myk OCA], [http://www.ebi.ac.uk/pdbsum/1myk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1myk RCSB]</span>
| + | [https://www.uniprot.org/uniprot/RARC_BPP22 RARC_BPP22] This protein acts as a transcriptional repressor of its own gene arc and of gene ant. |
| - | }}
| + | |
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| - | '''CRYSTAL STRUCTURE, FOLDING, AND OPERATOR BINDING OF THE HYPERSTABLE ARC REPRESSOR MUTANT PL8'''
| + | ==See Also== |
| - | | + | *[[Myosin 3D Structures|Myosin 3D Structures]] |
| - | | + | __TOC__ |
| - | ==Overview==
| + | </StructureSection> |
| - | Arc repressor is a small, dimeric DNA-binding protein that belongs to the ribbon-helix-helix family of transcription factors. Replacing Pro8 at the N-terminal end of the beta-sheet with leucine increases the stability of the mutant protein by 2.5 kcal/mol of dimer. However, this enhanced stability is achieved at the expense of significantly reduced DNA binding affinity. The structure of the PL8 mutant dimer has been determined to 2.4-A resolution by X-ray crystallography. The overall structure of the mutant is very similar to wild type, but Leu8 makes an additional interstrand hydrogen bond at each end of the beta-sheet of the mutant, increasing the total number of beta-sheet hydrogen bonds from six to eight. Comparison of the refolding and unfolding kinetics of the PL8 mutant and wild-type Arc shows that the enhanced stability of the mutant is accounted for by a decrease in the rate of protein unfolding, suggesting that the mutation acts to stabilize the native state and that the beta-sheet forms after the rate-limiting step in folding. The reduced operator affinity of the PL8 dimer appears to arise because the mutant cannot make the new interstrand hydrogen bonds and simultaneously make the wild-type set of contacts with operator DNA.
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Salmonella virus P22]] |
| - | ==About this Structure== | + | [[Category: Jeffrey PD]] |
| - | 1MYK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MYK OCA].
| + | [[Category: Milla ME]] |
| - | | + | [[Category: Raumann BE]] |
| - | ==Reference==
| + | [[Category: Sauer RT]] |
| - | Crystal structure, folding, and operator binding of the hyperstable Arc repressor mutant PL8., Schildbach JF, Milla ME, Jeffrey PD, Raumann BE, Sauer RT, Biochemistry. 1995 Jan 31;34(4):1405-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7827088 7827088]
| + | [[Category: Schildbach JF]] |
| - | [[Category: Enterobacteria phage p22]] | + | |
| - | [[Category: Single protein]] | + | |
| - | [[Category: Jeffrey, P D.]] | + | |
| - | [[Category: Milla, M E.]] | + | |
| - | [[Category: Raumann, B E.]] | + | |
| - | [[Category: Sauer, R T.]] | + | |
| - | [[Category: Schildbach, J F.]] | + | |
| - | [[Category: transcription regulation]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:22:54 2008''
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