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| | ==Molecular Mimicry of SUMO promotes DNA repair== | | ==Molecular Mimicry of SUMO promotes DNA repair== |
| - | <StructureSection load='3goe' size='340' side='right' caption='[[3goe]], [[Resolution|resolution]] 0.97Å' scene=''> | + | <StructureSection load='3goe' size='340' side='right'caption='[[3goe]], [[Resolution|resolution]] 0.97Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3goe]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_356 Cbs 356]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GOE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GOE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3goe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GOE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GOE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.97Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rad60, SPBC1921.02 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3goe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3goe OCA], [http://pdbe.org/3goe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3goe RCSB], [http://www.ebi.ac.uk/pdbsum/3goe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3goe ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3goe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3goe OCA], [https://pdbe.org/3goe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3goe RCSB], [https://www.ebi.ac.uk/pdbsum/3goe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3goe ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RAD60_SCHPO RAD60_SCHPO]] Required for repair of DNA double strand breaks which occur during replication, or induced by UV or gamma radiation, via recombination between sister chromatids. This has a subsequent role in the maintenance of chromosome structure. May work in conjunction with the Smc5-Smc6 complex.<ref>PMID:11971984</ref> <ref>PMID:12897162</ref> | + | [https://www.uniprot.org/uniprot/RAD60_SCHPO RAD60_SCHPO] Required for repair of DNA double strand breaks which occur during replication, or induced by UV or gamma radiation, via recombination between sister chromatids. This has a subsequent role in the maintenance of chromosome structure. May work in conjunction with the Smc5-Smc6 complex.<ref>PMID:11971984</ref> <ref>PMID:12897162</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> | | Check<jmol> |
| | <jmolCheckbox> | | <jmolCheckbox> |
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/go/3goe_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/go/3goe_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cbs 356]] | + | [[Category: Large Structures]] |
| - | [[Category: Perry, J J.P]] | + | [[Category: Schizosaccharomyces pombe]] |
| - | [[Category: Dna damage]] | + | [[Category: Perry JJP]] |
| - | [[Category: Dna recombination]]
| + | |
| - | [[Category: Dna repair]]
| + | |
| - | [[Category: Genome stability]]
| + | |
| - | [[Category: Nucleus]]
| + | |
| - | [[Category: Phosphoprotein]]
| + | |
| - | [[Category: Recombination]]
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| - | [[Category: Replication]]
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| - | [[Category: Sumo]]
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| - | [[Category: Sumo-like domain]]
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| - | [[Category: Sumoylation]]
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| Structural highlights
Function
RAD60_SCHPO Required for repair of DNA double strand breaks which occur during replication, or induced by UV or gamma radiation, via recombination between sister chromatids. This has a subsequent role in the maintenance of chromosome structure. May work in conjunction with the Smc5-Smc6 complex.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Rad60 family members contain functionally enigmatic, integral SUMO-like domains (SLDs). We show here that despite their divergence from SUMO, each Rad60 SLD interacts with a subset of SUMO pathway enzymes: SLD2 specifically binds the SUMO E2 conjugating enzyme (Ubc9), whereas SLD1 binds the SUMO E1 (Fub2, also called Uba2) activating and E3 (Pli1, also called Siz1 and Siz2) specificity enzymes. The molecular basis of this selectivity is revealed by our 0.97-A resolution crystal structure of Rad60 SLD2, which shows that apart from the conserved non-substrate SUMO:Ubc9 interface, the surface features of SLD2 are distinct from those of SUMO. Abrogation of the SLD2:Ubc9 FEG motif-dependent interaction results in hypersensitivity to genotoxic stress and an increase in spontaneous recombination associated with aberrant replication forks. Our results provide a mechanistic basis for the near-synonymous roles of Rad60 and SUMO in survival of genotoxic stress and suggest unprecedented DNA-damage-response functions for SLDs in regulating sumoylation.
Molecular mimicry of SUMO promotes DNA repair.,Prudden J, Perry JJ, Arvai AS, Tainer JA, Boddy MN Nat Struct Mol Biol. 2009 May;16(5):509-16. Epub 2009 Apr 12. PMID:19363481[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Morishita T, Tsutsui Y, Iwasaki H, Shinagawa H. The Schizosaccharomyces pombe rad60 gene is essential for repairing double-strand DNA breaks spontaneously occurring during replication and induced by DNA-damaging agents. Mol Cell Biol. 2002 May;22(10):3537-48. PMID:11971984
- ↑ Boddy MN, Shanahan P, McDonald WH, Lopez-Girona A, Noguchi E, Yates III JR, Russell P. Replication checkpoint kinase Cds1 regulates recombinational repair protein Rad60. Mol Cell Biol. 2003 Aug;23(16):5939-46. PMID:12897162
- ↑ Prudden J, Perry JJ, Arvai AS, Tainer JA, Boddy MN. Molecular mimicry of SUMO promotes DNA repair. Nat Struct Mol Biol. 2009 May;16(5):509-16. Epub 2009 Apr 12. PMID:19363481 doi:10.1038/nsmb.1582
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