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| | ==Coiled-Coil Domain-Dependent Homodimerization of Intracellular MLA Immune Receptors Defines a Minimal Functional Module for Triggering Cell Death== | | ==Coiled-Coil Domain-Dependent Homodimerization of Intracellular MLA Immune Receptors Defines a Minimal Functional Module for Triggering Cell Death== |
| - | <StructureSection load='3qfl' size='340' side='right' caption='[[3qfl]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='3qfl' size='340' side='right'caption='[[3qfl]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3qfl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Barley Barley]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QFL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QFL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3qfl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QFL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QFL FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.997Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mla10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4513 Barley])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qfl OCA], [http://pdbe.org/3qfl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qfl RCSB], [http://www.ebi.ac.uk/pdbsum/3qfl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qfl ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qfl OCA], [https://pdbe.org/3qfl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qfl RCSB], [https://www.ebi.ac.uk/pdbsum/3qfl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qfl ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8GSK4_HORVU Q8GSK4_HORVU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Barley]] | + | [[Category: Hordeum vulgare]] |
| - | [[Category: Chai, J]] | + | [[Category: Large Structures]] |
| - | [[Category: Cheng, W]] | + | [[Category: Chai J]] |
| - | [[Category: Coiled-coil]] | + | [[Category: Cheng W]] |
| - | [[Category: Leucine-rich repeat containing receptor]]
| + | |
| - | [[Category: Nlr]]
| + | |
| - | [[Category: Nucleotide-binding domain]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| Structural highlights
Function
Q8GSK4_HORVU
Publication Abstract from PubMed
Plants and animals have evolved structurally related innate immune sensors, designated NLRs, to detect intracellular nonself molecules. NLRs are modular, consisting of N-terminal coiled-coil (CC) or TOLL/interleukin-1 receptor (TIR) domains, a central nucleotide-binding (NB) domain, and C-terminal leucine-rich repeats (LRRs). The polymorphic barley mildew A (MLA) locus encodes CC-containing allelic immune receptors recognizing effectors of the pathogenic powdery mildew fungus. We report the crystal structure of an MLA receptor's invariant CC domain, which reveals a rod-shaped homodimer. MLA receptors also self-associate in vivo, but self-association appears to be independent of effector-triggered receptor activation. MLA CC mutants that fail to self-interact impair in planta cell death activity triggered by the CC domain alone and by an autoactive full-length MLA receptor that mimics its ATP-bound state. Thus, CC domain-dependent dimerization of the immune sensor defines a minimal functional unit and implies a role for the dimeric CC module in downstream immune signaling.
Coiled-coil domain-dependent homodimerization of intracellular barley immune receptors defines a minimal functional module for triggering cell death.,Maekawa T, Cheng W, Spiridon LN, Toller A, Lukasik E, Saijo Y, Liu P, Shen QH, Micluta MA, Somssich IE, Takken FL, Petrescu AJ, Chai J, Schulze-Lefert P Cell Host Microbe. 2011 Mar 17;9(3):187-99. PMID:21402358[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Maekawa T, Cheng W, Spiridon LN, Toller A, Lukasik E, Saijo Y, Liu P, Shen QH, Micluta MA, Somssich IE, Takken FL, Petrescu AJ, Chai J, Schulze-Lefert P. Coiled-coil domain-dependent homodimerization of intracellular barley immune receptors defines a minimal functional module for triggering cell death. Cell Host Microbe. 2011 Mar 17;9(3):187-99. PMID:21402358 doi:10.1016/j.chom.2011.02.008
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