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| | ==Nodamura virus protein b2, RNA-binding domain== | | ==Nodamura virus protein b2, RNA-binding domain== |
| - | <StructureSection load='3g80' size='340' side='right' caption='[[3g80]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='3g80' size='340' side='right'caption='[[3g80]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3g80]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Nodav Nodav]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3G80 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3g80]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nodamura_virus Nodamura virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G80 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3G80 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">B2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12288 NODAV])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3g80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g80 OCA], [http://pdbe.org/3g80 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3g80 RCSB], [http://www.ebi.ac.uk/pdbsum/3g80 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3g80 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3g80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g80 OCA], [https://pdbe.org/3g80 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3g80 RCSB], [https://www.ebi.ac.uk/pdbsum/3g80 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3g80 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/B2_NODAM B2_NODAM]] Binds double-strand RNA (dsRNA) with high affinity. Suppresses the host RNA silencing-based antiviral response.<ref>PMID:19249868</ref> | + | [https://www.uniprot.org/uniprot/B2_NODAM B2_NODAM] Binds double-strand RNA (dsRNA) with high affinity. Suppresses the host RNA silencing-based antiviral response.<ref>PMID:19249868</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Nodav]] | + | [[Category: Large Structures]] |
| - | [[Category: Ali, P Shaik Syed]] | + | [[Category: Nodamura virus]] |
| - | [[Category: Chen, J C]] | + | [[Category: Chen JC]] |
| - | [[Category: Korber, S]] | + | [[Category: Korber S]] |
| - | [[Category: Rna interference]] | + | [[Category: Shaik Syed Ali P]] |
| - | [[Category: Rna-binding]]
| + | |
| - | [[Category: Suppressor of rnai]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
B2_NODAM Binds double-strand RNA (dsRNA) with high affinity. Suppresses the host RNA silencing-based antiviral response.[1]
Publication Abstract from PubMed
Protein B2 from Nodamura virus (NMV B2), a member of the Nodavirus family, acts as a suppressor of RNA interference (RNAi). The N-terminal domain of NMV B2, consisting of residues 1-79, recognizes double-stranded RNA (dsRNA). The 2.5 A crystal structure of the RNA-binding domain of NMV B2 shows a dimeric, helical bundle structure. The structure shows a conserved set of RNA-binding residues compared with flock house virus B2, despite limited sequence identity. The crystal packing places the RNA-binding residues along one face of symmetry-related molecules, suggesting a potential platform for recognition of dsRNA.
Structure of the RNA-Binding Domain of Nodamura Virus Protein B2, a Suppressor of RNA Interference (dagger) , (double dagger).,Korber S, Shaik Syed Ali P, Chen JC Biochemistry. 2009 Mar 24;48(11):2307-9. PMID:19249868[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Korber S, Shaik Syed Ali P, Chen JC. Structure of the RNA-Binding Domain of Nodamura Virus Protein B2, a Suppressor of RNA Interference (dagger) , (double dagger). Biochemistry. 2009 Mar 24;48(11):2307-9. PMID:19249868 doi:10.1021/bi900126s
- ↑ Korber S, Shaik Syed Ali P, Chen JC. Structure of the RNA-Binding Domain of Nodamura Virus Protein B2, a Suppressor of RNA Interference (dagger) , (double dagger). Biochemistry. 2009 Mar 24;48(11):2307-9. PMID:19249868 doi:10.1021/bi900126s
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