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| | ==The crystal structure of isomaltulose synthase mutant E295Q from Erwinia rhapontici NX5 in complex with its natural substrate sucrose== | | ==The crystal structure of isomaltulose synthase mutant E295Q from Erwinia rhapontici NX5 in complex with its natural substrate sucrose== |
| - | <StructureSection load='4hph' size='340' side='right' caption='[[4hph]], [[Resolution|resolution]] 1.70Å' scene=''> | + | <StructureSection load='4hph' size='340' side='right'caption='[[4hph]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4hph]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"aplanobacter_rhaponticum"_(sic)_(millard_1924)_white_1936 "aplanobacter rhaponticum" (sic) (millard 1924) white 1936]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HPH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HPH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4hph]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Erwinia_rhapontici Erwinia rhapontici]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HPH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HPH FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4how|4how]], [[4hox|4hox]], [[4hoz|4hoz]], [[4hp5|4hp5]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PalI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=55212 "Aplanobacter rhaponticum" (sic) (Millard 1924) White 1936])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hph OCA], [https://pdbe.org/4hph PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hph RCSB], [https://www.ebi.ac.uk/pdbsum/4hph PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hph ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isomaltulose_synthase Isomaltulose synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.11 5.4.99.11] </span></td></tr> | + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hph OCA], [http://pdbe.org/4hph PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hph RCSB], [http://www.ebi.ac.uk/pdbsum/4hph PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hph ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/D9MPF2_ERWRD D9MPF2_ERWRD] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4hph" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4hph" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Trehalulose synthase|Trehalulose synthase]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Isomaltulose synthase]] | + | [[Category: Erwinia rhapontici]] |
| - | [[Category: Li, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Xu, H]] | + | [[Category: Li S]] |
| - | [[Category: Xu, Z]] | + | [[Category: Xu H]] |
| - | [[Category: Zhou, J]] | + | [[Category: Xu Z]] |
| - | [[Category: Calcium binding]]
| + | [[Category: Zhou J]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Isomerase]]
| + | |
| - | [[Category: Tim barrel]]
| + | |
| Structural highlights
4hph is a 1 chain structure with sequence from Erwinia rhapontici. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 1.7Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
D9MPF2_ERWRD
Publication Abstract from PubMed
Sucrose isomerase NX-5 from Erwiniarhapontici efficiently catalyzes the isomerization of sucrose to isomaltulose (main product) and trehalulose (by-product). To investigate the molecular mechanism controlling sucrose isomer formation, we determined the crystal structures of native NX-5 and its mutant complexes E295Q/sucrose and D241A/glucose at 1.70 A, 1.70 A and 2.00 A, respectively. The overall structure and active site architecture of NX-5 resemble those of other reported sucrose isomerases. Strikingly, the substrate binding mode of NX-5 is also similar to that of trehalulose synthase from Pseudomonasmesoacidophila MX-45 (MutB). Detailed structural analysis revealed the catalytic RXDRX motif and the adjacent 10-residue loop of NX-5 and isomaltulose synthase PalI from Klebsiella sp. LX3 adopt a distinct orientation from those of trehalulose synthases. Mutations of the loop region of NX-5 resulted in significant changes of the product ratio between isomaltulose and trehalulose. The molecular dynamics simulation data supported the product specificity of NX-5 towards isomaltulose and the role of the loop(330-339) in NX-5 catalysis. This work should prove useful for the engineering of sucrose isomerase for industrial carbohydrate biotransformations.
The Structural Basis of Erwinia rhapontici Isomaltulose Synthase.,Xu Z, Li S, Li J, Li Y, Feng X, Wang R, Xu H, Zhou J PLoS One. 2013 Sep 19;8(9):e74788. doi: 10.1371/journal.pone.0074788. PMID:24069347[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Xu Z, Li S, Li J, Li Y, Feng X, Wang R, Xu H, Zhou J. The Structural Basis of Erwinia rhapontici Isomaltulose Synthase. PLoS One. 2013 Sep 19;8(9):e74788. doi: 10.1371/journal.pone.0074788. PMID:24069347 doi:http://dx.doi.org/10.1371/journal.pone.0074788
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