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1n21

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[[Image:1n21.gif|left|200px]]
 
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{{Structure
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==(+)-Bornyl Diphosphate Synthase: Cocrystal with Mg and 3-aza-2,3-dihydrogeranyl diphosphate==
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|PDB= 1n21 |SIZE=350|CAPTION= <scene name='initialview01'>1n21</scene>, resolution 3.10&Aring;
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<StructureSection load='1n21' size='340' side='right'caption='[[1n21]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=3AG:2-[METHYL-(4-METHYL-PENT-3-ENYL)-AMINO]-ETHYL-DIPHOSPHATE'>3AG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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<table><tr><td colspan='2'>[[1n21]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salvia_officinalis Salvia officinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N21 FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Geranyl-diphosphate_cyclase Geranyl-diphosphate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.8 5.5.1.8] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3AG:2-[METHYL-(4-METHYL-PENT-3-ENYL)-AMINO]-ETHYL-DIPHOSPHATE'>3AG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n21 OCA], [https://pdbe.org/1n21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n21 RCSB], [https://www.ebi.ac.uk/pdbsum/1n21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n21 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1n1b|1N1B]], [[1n1z|1N1Z]], [[1n20|1N20]], [[1n22|1N22]], [[1n23|1N23]], [[1n24|1N24]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n21 OCA], [http://www.ebi.ac.uk/pdbsum/1n21 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n21 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/BPPS_SALOF BPPS_SALOF] Catalyzes the formation of the (+)-camphor precursor (+)-bornyl diphosphate from geranyl diphosphate. The enzyme also produces significant amounts of (+)-alpha-pinene, (+)-camphene, and (+-)-limonene.<ref>PMID:9614092</ref>
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== Evolutionary Conservation ==
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'''(+)-Bornyl Diphosphate Synthase: Cocrystal with Mg and 3-aza-2,3-dihydrogeranyl diphosphate'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n2/1n21_consurf.spt"</scriptWhenChecked>
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The x-ray crystal structure of dimeric (+)-bornyl diphosphate synthase, a metal-requiring monoterpene cyclase from Salvia officinalis, is reported at 2.0-A resolution. Each monomer contains two alpha-helical domains: the C-terminal domain catalyzes the cyclization of geranyl diphosphate, orienting and stabilizing multiple reactive carbocation intermediates; the N-terminal domain has no clearly defined function, although its N terminus caps the active site in the C-terminal domain during catalysis. Structures of complexes with aza analogues of substrate and carbocation intermediates, as well as complexes with pyrophosphate and bornyl diphosphate, provide "snapshots" of the terpene cyclization cascade.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1N21 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salvia_officinalis Salvia officinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N21 OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n21 ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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Bornyl diphosphate synthase: structure and strategy for carbocation manipulation by a terpenoid cyclase., Whittington DA, Wise ML, Urbansky M, Coates RM, Croteau RB, Christianson DW, Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15375-80. Epub 2002 Nov 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12432096 12432096]
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<references/>
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[[Category: Geranyl-diphosphate cyclase]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Salvia officinalis]]
[[Category: Salvia officinalis]]
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[[Category: Single protein]]
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[[Category: Christianson DW]]
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[[Category: Christianson, D W.]]
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[[Category: Coates RM]]
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[[Category: Coates, R M.]]
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[[Category: Croteau RB]]
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[[Category: Croteau, R B.]]
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[[Category: Urbansky M]]
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[[Category: Urbansky, M.]]
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[[Category: Whittington DA]]
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[[Category: Whittington, D A.]]
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[[Category: Wise ML]]
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[[Category: Wise, M L.]]
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[[Category: terpene synthase fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:24:14 2008''
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Current revision

(+)-Bornyl Diphosphate Synthase: Cocrystal with Mg and 3-aza-2,3-dihydrogeranyl diphosphate

PDB ID 1n21

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