4j2c

<StructureSection load='4j2c' size='340' side='right' caption='[[4j2c]], [[Resolution|resolution]] 1.80&Aring;' scene=''>

<table><tr><td colspan='2'>[[4j2c]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J2C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J2C FirstGlance]. <br>

</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">STX6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j2c OCA], [http://pdbe.org/4j2c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j2c RCSB], [http://www.ebi.ac.uk/pdbsum/4j2c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j2c ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/STX6_HUMAN STX6_HUMAN]] Involved in intracellular vesicle trafficking. [[http://www.uniprot.org/uniprot/VPS51_HUMAN VPS51_HUMAN]] Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi networkl (TGN). The GARP complex is required for the maintenance of protein retrieval from endosomes to the TGN, acid hydrolase sorting, lysosome function, endosomal cholesterol traffic and autophagy. VPS51 participates in retrograde transport of acid hydrolase receptors, likely by promoting tethering and SNARE-dependent fusion of endosome-derived carriers to the TGN.<ref>PMID:20685960</ref>

The Golgi-Associated Retrograde Protein (GARP) complex is a tethering factor involved in the fusion of endosome-derived transport vesicles to the trans-Golgi network through interaction with components of the Syntaxin 6/Syntaxin 16/Vti1a/VAMP4 SNARE complex. The mechanisms by which GARP and other tethering factors engage the SNARE fusion machinery are poorly understood. Herein, we report the structural basis for the interaction of the human Ang2 subunit of GARP with the Syntaxin 6 and the closely related Syntaxin 10. The crystal structure of the Syntaxin 6 Habc domain in complex with a peptide from the N terminus of Ang2 shows a binding mode in which a dityrosine motif of Ang2 interacts with a highly conserved groove in Syntaxin 6. Structure-based mutational analyses validate the crystal structure and support the phylogenetic conservation of this interaction.

 

<div class="pdbe-citations 4j2c" style="background-color:#fffaf0;"></div>

== References ==

[[Category: Human]]

[[Category: Abascal-Palacios, G]]

[[Category: Bonifacino, J S]]

[[Category: Hierro, A]]

[[Category: Rojas, A L]]

[[Category: Schindler, C]]

[[Category: Tethering complex]]