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| | ==Crystal structure of bacterial cell-surface alginate-binding protein Algp7== | | ==Crystal structure of bacterial cell-surface alginate-binding protein Algp7== |
| - | <StructureSection load='3at7' size='340' side='right' caption='[[3at7]], [[Resolution|resolution]] 2.10Å' scene=''> | + | <StructureSection load='3at7' size='340' side='right'caption='[[3at7]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3at7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_sp._a1 Sphingomonas sp. a1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AT7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AT7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3at7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingomonas_sp._A1 Sphingomonas sp. A1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AT7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AT7 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">p7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90322 Sphingomonas sp. A1])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3at7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3at7 OCA], [http://pdbe.org/3at7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3at7 RCSB], [http://www.ebi.ac.uk/pdbsum/3at7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3at7 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3at7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3at7 OCA], [https://pdbe.org/3at7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3at7 RCSB], [https://www.ebi.ac.uk/pdbsum/3at7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3at7 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q25C86_9SPHN Q25C86_9SPHN] |
| - | A gram-negative Sphingomonas sp. A1 directly incorporates alginate polysaccharide into the cytoplasm via the cell-surface pit and ABC transporter. A cell-surface alginate-binding protein, Algp7, functions as a concentrator of the polysaccharide in the pit. Based on the primary structure and genetic organization in the bacterial genome, Algp7 was found to be homologous to an M75 peptidase motif-containing EfeO, a component of a ferrous ion transporter. Despite the presence of an M75 peptidase motif with high similarity, the Algp7 protein purified from recombinant Escherichia coli cells was inert on insulin B chain and N-benzoyl-Phe-Val-Arg-p-nitroanilide, both of which are substrates for a typical M75 peptidase, imelysin, from Pseudomonas aeruginosa. The X-ray crystallographic structure of Algp7 was determined at 2.10A resolution by single-wavelength anomalous diffraction. Although a metal-binding motif, HxxE, conserved in zinc ion-dependent M75 peptidases is also found in Algp7, the crystal structure of Algp7 contains no metal even at the motif. The protein consists of two structurally similar up-and-down helical bundles as the basic scaffold. A deep cleft between the bundles is sufficiently large to accommodate macromolecules such as alginate polysaccharide. This is the first structural report on a bacterial cell-surface alginate-binding protein with an M75 peptidase motif.
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| - | Crystal structure of bacterial cell-surface alginate-binding protein with an M75 peptidase motif.,Maruyama Y, Ochiai A, Mikami B, Hashimoto W, Murata K Biochem Biophys Res Commun. 2011 Feb 18;405(3):411-6. Epub 2011 Jan 14. PMID:21238429<ref>PMID:21238429</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 3at7" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Sphingomonas sp. a1]] | + | [[Category: Large Structures]] |
| - | [[Category: Hashimoto, W]] | + | [[Category: Sphingomonas sp. A1]] |
| - | [[Category: Maruyama, Y]] | + | [[Category: Hashimoto W]] |
| - | [[Category: Mikami, B]] | + | [[Category: Maruyama Y]] |
| - | [[Category: Murata, K]] | + | [[Category: Mikami B]] |
| - | [[Category: Ochiai, A]] | + | [[Category: Murata K]] |
| - | [[Category: Alginate]]
| + | [[Category: Ochiai A]] |
| - | [[Category: Alginate binding]]
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| - | [[Category: Structural protein]]
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| - | [[Category: Two up-and-down four-helical bundle]]
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