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1n2z

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[[Image:1n2z.gif|left|200px]]
 
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{{Structure
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==2.0 Angstrom structure of BtuF, the vitamin B12 binding protein of E. coli==
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|PDB= 1n2z |SIZE=350|CAPTION= <scene name='initialview01'>1n2z</scene>, resolution 2.0&Aring;
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<StructureSection load='1n2z' size='340' side='right'caption='[[1n2z]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CNC:CO-CYANOCOBALAMIN'>CNC</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>
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<table><tr><td colspan='2'>[[1n2z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N2Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N2Z FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE= btuf ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CNC:CO-CYANOCOBALAMIN'>CNC</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n2z OCA], [https://pdbe.org/1n2z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n2z RCSB], [https://www.ebi.ac.uk/pdbsum/1n2z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n2z ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n2z OCA], [http://www.ebi.ac.uk/pdbsum/1n2z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n2z RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/BTUF_ECOLI BTUF_ECOLI] Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC.<ref>PMID:11790740</ref>
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== Evolutionary Conservation ==
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'''2.0 Angstrom structure of BtuF, the vitamin B12 binding protein of E. coli'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n2/1n2z_consurf.spt"</scriptWhenChecked>
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Bacterial binding protein-dependent ATP binding cassette (ABC) transporters facilitate uptake of essential nutrients. The crystal structure of Escherichia coli BtuF, the protein that binds vitamin B12 and delivers it to the periplasmic surface of the ABC transporter BtuCD, reveals a bi-lobed fold resembling that of the ferrichrome binding protein FhuD. B12 is bound in the "base-on" conformation in a deep cleft formed at the interface between the two lobes of BtuF. A stable complex between BtuF and BtuCD (with the stoichiometry BtuC2D2F) is demonstrated to form in vitro and was modeled using the individual crystal structures. Two surface glutamates from BtuF may interact with arginine residues on the periplasmic surface of the BtuCD transporter. These glutamate and arginine residues are conserved among binding proteins and ABC transporters mediating iron and B12 uptake, suggesting that they may have a role in docking and the transmission of conformational changes.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1N2Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N2Z OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n2z ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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The structure of Escherichia coli BtuF and binding to its cognate ATP binding cassette transporter., Borths EL, Locher KP, Lee AT, Rees DC, Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16642-7. Epub 2002 Dec 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12475936 12475936]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Borths, E L.]]
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[[Category: Borths EL]]
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[[Category: Lee, A T.]]
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[[Category: Lee AT]]
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[[Category: Locher, K P.]]
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[[Category: Locher KP]]
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[[Category: Rees, D C.]]
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[[Category: Rees DC]]
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[[Category: transport protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:24:37 2008''
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Current revision

2.0 Angstrom structure of BtuF, the vitamin B12 binding protein of E. coli

PDB ID 1n2z

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