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| | ==Crystal structure of far-red fluorescent protein Katushka crystallized at pH 8.5== | | ==Crystal structure of far-red fluorescent protein Katushka crystallized at pH 8.5== |
| - | <StructureSection load='3pj7' size='340' side='right' caption='[[3pj7]], [[Resolution|resolution]] 1.85Å' scene=''> | + | <StructureSection load='3pj7' size='340' side='right'caption='[[3pj7]], [[Resolution|resolution]] 1.85Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3pj7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bubble-tip_anemone Bubble-tip anemone]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PJ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PJ7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3pj7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Entacmaea_quadricolor Entacmaea quadricolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PJ7 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3bx9|3bx9]], [[3bxa|3bxa]], [[3bxb|3bxb]], [[3bxc|3bxc]], [[3pib|3pib]], [[3pj5|3pj5]], [[3pjb|3pjb]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pj7 OCA], [http://pdbe.org/3pj7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pj7 RCSB], [http://www.ebi.ac.uk/pdbsum/3pj7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pj7 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pj7 OCA], [https://pdbe.org/3pj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pj7 RCSB], [https://www.ebi.ac.uk/pdbsum/3pj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pj7 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/H3JQU6_ENTQU H3JQU6_ENTQU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Green Fluorescent Protein|Green Fluorescent Protein]] | + | *[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bubble-tip anemone]] | + | [[Category: Entacmaea quadricolor]] |
| - | [[Category: Pletnev, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Pletnev, V Z]] | + | [[Category: Pletnev S]] |
| - | [[Category: Pletneva, N V]] | + | [[Category: Pletnev VZ]] |
| - | [[Category: Beta-barrel]] | + | [[Category: Pletneva NV]] |
| - | [[Category: Biomarker]]
| + | |
| - | [[Category: Far-red fluorescent protein]]
| + | |
| - | [[Category: Fluorescent protein]]
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| - | [[Category: Katushka]]
| + | |
| - | [[Category: Met-tyr-gly chromophore]]
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| - | [[Category: Mutant variant of eqfp578]]
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| Structural highlights
Function
H3JQU6_ENTQU
Publication Abstract from PubMed
The wild type red fluorescent protein eqFP578 (from sea anemone Entacmaea quadricolor, lambdaex = 552 nm, lambdaem = 578 nm) and its bright far-red fluorescent variant Katushka (lambdaex = 588 nm, lambdaem = 635 nm) are characterized by the pronounced pH dependence of their fluorescence. The crystal structures of eqFP578f (eqFP578 with two point mutations improving the protein folding) and Katushka have been determined at the resolution ranging from 1.15 to 1.85 A at two pH values, corresponding to low and high level of fluorescence. The observed extinguishing of fluorescence upon reducing pH in eqFP578f and Katushka has been shown to be accompanied by the opposite trans-cis and cis-trans chromophore isomerization, respectively. Asn143, Ser158, His197 and Ser143, Leu174, and Arg197 have been shown to stabilize the respective trans and cis fluorescent states of the chromophores in eqFP578f and Katushka at higher pH. The cis state has been suggested as being primarily responsible for the observed far-red shift of the emission maximum of Katushka relative to that of eqFP578f.
Crystallographic study of red fluorescent protein eqFP578 and its far-red variant Katushka reveals opposite pH-induced isomerization of chromophore.,Pletneva NV, Pletnev VZ, Shemiakina II, Chudakov DM, Artemyev I, Wlodawer A, Dauter Z, Pletnev S Protein Sci. 2011 May 11. doi: 10.1002/pro.654. PMID:21563226[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Pletneva NV, Pletnev VZ, Shemiakina II, Chudakov DM, Artemyev I, Wlodawer A, Dauter Z, Pletnev S. Crystallographic study of red fluorescent protein eqFP578 and its far-red variant Katushka reveals opposite pH-induced isomerization of chromophore. Protein Sci. 2011 May 11. doi: 10.1002/pro.654. PMID:21563226 doi:10.1002/pro.654
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