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| | ==Crystal structure of Lumazine synthase from Salmonella typhimurium LT2== | | ==Crystal structure of Lumazine synthase from Salmonella typhimurium LT2== |
| - | <StructureSection load='3mk3' size='340' side='right' caption='[[3mk3]], [[Resolution|resolution]] 3.57Å' scene=''> | + | <StructureSection load='3mk3' size='340' side='right'caption='[[3mk3]], [[Resolution|resolution]] 3.57Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3mk3]] is a 60 chain structure with sequence from [http://en.wikipedia.org/wiki/Salty Salty]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MK3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MK3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3mk3]] is a 60 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MK3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MK3 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.569Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ribH, STM0417 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 SALTY])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6,7-dimethyl-8-ribityllumazine_synthase 6,7-dimethyl-8-ribityllumazine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.78 2.5.1.78] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mk3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mk3 OCA], [https://pdbe.org/3mk3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mk3 RCSB], [https://www.ebi.ac.uk/pdbsum/3mk3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mk3 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mk3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mk3 OCA], [http://pdbe.org/3mk3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3mk3 RCSB], [http://www.ebi.ac.uk/pdbsum/3mk3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3mk3 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RISB_SALTY RISB_SALTY]] Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin (By similarity). | + | [https://www.uniprot.org/uniprot/RISB_SALTY RISB_SALTY] Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: 6,7-dimethyl-8-ribityllumazine synthase]] | + | [[Category: Large Structures]] |
| - | [[Category: Salty]] | + | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]] |
| - | [[Category: Karthikeyan, S]] | + | [[Category: Karthikeyan S]] |
| - | [[Category: Kumar, P]] | + | [[Category: Kumar P]] |
| - | [[Category: Singh, M]] | + | [[Category: Singh M]] |
| - | [[Category: Dmrl synthase]]
| + | |
| - | [[Category: Drug target]]
| + | |
| - | [[Category: Flavodoxin like fold]]
| + | |
| - | [[Category: Icosahedral]]
| + | |
| - | [[Category: Riboflavin biosynthesis]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
RISB_SALTY Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin (By similarity).
Publication Abstract from PubMed
Riboflavin biosynthesis is an essential pathway in bacteria, in contrast to animals, which obtain riboflavin from their diet. Therefore, the enzymes involved in the riboflavin-biosynthesis pathway are potential targets for the development of antibacterial drugs. Lumazine synthase, an enzyme that is involved in the penultimate step of riboflavin biosynthesis, catalyzes the formation of 6,7-dimethyl-8-ribityllumazine from 3,4-dihydroxy-2-butanone 4-phosphate and 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione. Lumazine synthase from Salmonella typhimurium (sLS) has been cloned, overexpressed, purified and was crystallized in three forms, each with different crystal packing. The crystal structure of sLS in the monoclinic space group P2(1) has been determined with 60 subunits per asymmetric unit, packed as an icosahedron, at 3.57 A resolution. Interestingly, sLS contains an N-terminal proline residue (Pro11) which had previously been suggested to disrupt the formation of the icosohedral assembly. In addition, comparison of the structure of sLS with known orthologous lumazine synthase structures allowed identification of the amino-acid residues involved in substrate binding and catalysis. The sLS structure reported here could serve as a starting point for the development of species-specific antibacterial drugs.
Crystal structure analysis of icosahedral lumazine synthase from Salmonella typhimurium, an antibacterial drug target.,Kumar P, Singh M, Karthikeyan S Acta Crystallogr D Biol Crystallogr. 2011 Feb;67(Pt 2):131-9. Epub 2011, Jan 15. PMID:21245535[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kumar P, Singh M, Karthikeyan S. Crystal structure analysis of icosahedral lumazine synthase from Salmonella typhimurium, an antibacterial drug target. Acta Crystallogr D Biol Crystallogr. 2011 Feb;67(Pt 2):131-9. Epub 2011, Jan 15. PMID:21245535 doi:10.1107/S0907444910053370
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