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| | ==Crystal structure of human alpha-defensin 1, HNP1 (G17A mutant)== | | ==Crystal structure of human alpha-defensin 1, HNP1 (G17A mutant)== |
| - | <StructureSection load='4du0' size='340' side='right' caption='[[4du0]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='4du0' size='340' side='right'caption='[[4du0]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4du0]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DU0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DU0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4du0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DU0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3gny|3gny]], [[3hj2|3hj2]], [[3hjd|3hjd]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4du0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4du0 OCA], [http://pdbe.org/4du0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4du0 RCSB], [http://www.ebi.ac.uk/pdbsum/4du0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4du0 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4du0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4du0 OCA], [https://pdbe.org/4du0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4du0 RCSB], [https://www.ebi.ac.uk/pdbsum/4du0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4du0 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/DEF1_HUMAN DEF1_HUMAN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Defensin|Defensin]] | + | *[[Defensin 3D structures|Defensin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lu, W]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Pazgier, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Wu, X]] | + | [[Category: Lu W]] |
| - | [[Category: Alpha-defensin]] | + | [[Category: Pazgier M]] |
| - | [[Category: Antibiotic]] | + | [[Category: Wu X]] |
| - | [[Category: Antimicrobial peptide]]
| + | |
| - | [[Category: Cysteine rich antimicrobial peptide]]
| + | |
| - | [[Category: Defensin fold]]
| + | |
| - | [[Category: G17a mutant]]
| + | |
| - | [[Category: Hnp1]]
| + | |
| - | [[Category: Human alpha-defensin 1]]
| + | |
| - | [[Category: Human neutrophil]]
| + | |
| Structural highlights
Function
DEF1_HUMAN
Publication Abstract from PubMed
The human alpha-defensins are synthesized in vivo as inactive pro-defensins, and contain a conserved glycine, Gly-17, which is part of a beta-bulge structure. It had previously been shown that the glycine main-chain torsion angles are in a D-configuration, and that D-amino acids but not L-alanine could be substituted at that position to yield correctly folded peptides without the help of a pro domain. In this study, the glycine to L-alanine mutant defensin was synthesized in the form of a pro-defensin using native chemical ligation. The ligation product folded correctly and yielded an active peptide upon CNBr cleavage. The L-Ala-17-HNP1 crystal structure depicted a beta-bulge identical to wild-type HNP1. However, dimerization was perturbed, causing one monomer to tilt with respect to the other in a dimerization model. Inhibitory activity against anthrax lethal factor (LF) showed a two-fold reduction relative to the wild-type HNP1 as measured by the inhibitory concentration IC50. Self-association was slightly reduced, as detected by surface plasmon resonance (SPR) measurements. According to the results of the virtual colony count (vCC) assay, the antibacterial activity against Escherichia coli, Staphylococcus aureus, and Bacillus cereus exhibited a less than two-fold reduction in virtual lethal dose values. Pro-defensins with two other L-amino acid substitutions, Arg and Phe, at the same position did not fold, indicating that only small side chains are tolerable. These results further elucidate the factors governing the region of the beta-bulge structure that includes Gly-17, illuminating why glycine is conserved in all mammalian alpha-defensins.
The invariant Gly residue is important for alpha defensin folding, dimerization and function: a case study of the human neutrophil alpha defensin HNP1.,Zhao L, Ericksen B, Wu X, Zhan C, Yuan W, Li X, Pazgier M, Lu W J Biol Chem. 2012 Apr 11. PMID:22496447[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Zhao L, Ericksen B, Wu X, Zhan C, Yuan W, Li X, Pazgier M, Lu W. The invariant Gly residue is important for alpha defensin folding, dimerization and function: a case study of the human neutrophil alpha defensin HNP1. J Biol Chem. 2012 Apr 11. PMID:22496447 doi:10.1074/jbc.M112.355255
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