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3zh3

From Proteopedia

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crystal structure of S. pneumoniae D39 native MurA1

Structural highlights

3zh3 is a 1 chain structure with sequence from Streptococcus pneumoniae D39. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.9Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MURA1_STRP2 Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine (By similarity). Target for the antibiotic fosfomycin. Involved in heteroresistance to antibiotic fosfomycin. Heteroresistance is the ability of a clonal population to grow one or several subpopulations at a frequency of 10(-7) to 10(-3) in the presence of a higher antibiotic concentration than that predicted to be effective by measurement of the minimum inhibitory concentration (MIC) (PubMed:23571543).[HAMAP-Rule:MF_00111]^[1]

Publication Abstract from PubMed

Fosfomycin targets the first step of peptidoglycan biosynthesis in Streptococcus pneumoniae catalyzed by UDP-N-acetylglucosamine enolpyruvyltransferase (MurA1). We investigated whether heteroresistance to fosfomycin occurs in S. pneumoniae. We found that of 11 strains tested all but one (Hungary19A) displayed heteroresistance and that deletion of murA1 abolished heteroresistance. Hungary19A differs from the other strains by a single amino-acid substitution in MurA1 (Ala364Thr). To test whether this substitution is responsible for lack of heteroresistance, it was introduced into strain D39. The heteroresistant phenotype of strain D39 was not changed. Furthermore, no relevant structural differences between MurA1 of the heteroresistant strain D39 or the non-heteroresistant strain Hungary19A were found in their crystal structures. Our results reveal that heteroresistance to fosfomycin is the predominant phenotype in S. pneumoniae and that MurA1 is required for heteroresistance to fosfomycin but is not the only factor involved. The findings provide a caveat for any future use of fosfomycin in the treatment of pneumococcal infections.

Heteroresistance to fosfomycin is predominant in Streptococcus pneumoniae and depends on murA1 gene.,Engel H, Gutierrez-Fernandez J, Fluckiger C, Martinez-Ripoll M, Muhlemann K, Hermoso JA, Hilty M, Hathaway LJ Antimicrob Agents Chemother. 2013 Apr 9. PMID:23571543^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Engel H, Gutierrez-Fernandez J, Fluckiger C, Martinez-Ripoll M, Muhlemann K, Hermoso JA, Hilty M, Hathaway LJ. Heteroresistance to fosfomycin is predominant in Streptococcus pneumoniae and depends on murA1 gene. Antimicrob Agents Chemother. 2013 Apr 9. PMID:23571543 doi:10.1128/AAC.00223-13
↑ Engel H, Gutierrez-Fernandez J, Fluckiger C, Martinez-Ripoll M, Muhlemann K, Hermoso JA, Hilty M, Hathaway LJ. Heteroresistance to fosfomycin is predominant in Streptococcus pneumoniae and depends on murA1 gene. Antimicrob Agents Chemother. 2013 Apr 9. PMID:23571543 doi:10.1128/AAC.00223-13

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3zh3"

Categories: Large Structures | Streptococcus pneumoniae D39 | Gutierrez-Fernandez J | Hermoso JA

@@ Line 1: / Line 1: @@
 ==crystal structure of S. pneumoniae D39 native MurA1==
-<StructureSection load='3zh3' size='340' side='right' caption='[[3zh3]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
+<StructureSection load='3zh3' size='340' side='right'caption='[[3zh3]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3zh3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strp2 Strp2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZH3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZH3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3zh3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_D39 Streptococcus pneumoniae D39]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZH3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZH3 FirstGlance]. <br>
-</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_1-carboxyvinyltransferase UDP-N-acetylglucosamine 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.7 2.5.1.7] </span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zh3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zh3 OCA], [http://pdbe.org/3zh3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zh3 RCSB], [http://www.ebi.ac.uk/pdbsum/3zh3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zh3 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zh3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zh3 OCA], [https://pdbe.org/3zh3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zh3 RCSB], [https://www.ebi.ac.uk/pdbsum/3zh3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zh3 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/Q04KK5_STRP2 Q04KK5_STRP2]] Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine (By similarity).[HAMAP-Rule:MF_00111][SAAS:SAAS005750_004_003895]
+[https://www.uniprot.org/uniprot/MURA1_STRP2 MURA1_STRP2] Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine (By similarity). Target for the antibiotic fosfomycin. Involved in heteroresistance to antibiotic fosfomycin. Heteroresistance is the ability of a clonal population to grow one or several subpopulations at a frequency of 10(-7) to 10(-3) in the presence of a higher antibiotic concentration than that predicted to be effective by measurement of the minimum inhibitory concentration (MIC) (PubMed:23571543).[HAMAP-Rule:MF_00111]<ref>PMID:23571543</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 20: @@
 ==See Also==
-*[[Enoylpyruvate transferase|Enoylpyruvate transferase]]
+*[[Enoylpyruvate transferase 3D structures|Enoylpyruvate transferase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Strp2]]
+[[Category: Large Structures]]
-[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]
+[[Category: Streptococcus pneumoniae D39]]
-[[Category: Gutierrez-Fernandez, J]]
+[[Category: Gutierrez-Fernandez J]]
-[[Category: Hermoso, J A]]
+[[Category: Hermoso JA]]
-[[Category: Enolpyruvyl transferase]]
-[[Category: Mura]]
-[[Category: Transferase]]

3zh3

From Proteopedia

Current revision

crystal structure of S. pneumoniae D39 native MurA1

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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