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| - | [[Image:1n6t.jpg|left|200px]] | |
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| - | {{Structure
| + | ==Solution Structure of the Tachykinin Peptide Neurokinin A== |
| - | |PDB= 1n6t |SIZE=350|CAPTION= <scene name='initialview01'>1n6t</scene>
| + | <StructureSection load='1n6t' size='340' side='right'caption='[[1n6t]]' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND=
| + | <table><tr><td colspan='2'>[[1n6t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N6T FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | |GENE= | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n6t OCA], [https://pdbe.org/1n6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n6t RCSB], [https://www.ebi.ac.uk/pdbsum/1n6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n6t ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | == Function == |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n6t OCA], [http://www.ebi.ac.uk/pdbsum/1n6t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n6t RCSB]</span>
| + | [https://www.uniprot.org/uniprot/TKNB_CHICK TKNB_CHICK] Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. |
| - | }}
| + | __TOC__ |
| - | | + | </StructureSection> |
| - | '''Solution Structure of the Tachykinin Peptide Neurokinin A'''
| + | [[Category: Gallus gallus]] |
| - | | + | [[Category: Large Structures]] |
| - | | + | [[Category: Chandrashekar IR]] |
| - | ==Overview==
| + | [[Category: Cowsik SM]] |
| - | The solution structure of NKA, a decapeptide of mammalian origin, has been characterized by CD spectropolarimetry and 2D proton nuclear magnetic resonance (2D 1H-NMR) spectroscopy in both aqueous and membrane mimetic solvents. Unambiguous NMR assignments of protons have been made with the aid of correlation spectroscopy (DQF-COSY and TOCSY) experiments and nuclear Overhauser effect spectroscopy (NOESY and ROESY) experiments. The distance constraints obtained from the NMR data have been utilized to generate a family of structures, which have been refined using restrained energy minimization and dynamics. These data show that in water NKA prefers to be in an extended chain conformation whereas a helical conformation is induced in the central core and the C-terminal region (D4-M10) of the peptide in the presence of perdeuterated dodecylphosphocholine (DPC) micelles, a membrane model system. Though less defined the N-terminus also displays some degree of order and a possible turn structure. The conformation adopted by NKA in the presence of DPC micelles represents a structural motif typical of neurokinin-2 selective agonists and is similar to that reported for eledoisin in hydrophobic environment.
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| - | ==About this Structure==
| + | |
| - | 1N6T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N6T OCA].
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| - | | + | |
| - | ==Reference== | + | |
| - | Three-dimensional structure of the mammalian tachykinin peptide neurokinin A bound to lipid micelles., Chandrashekar IR, Cowsik SM, Biophys J. 2003 Dec;85(6):4002-11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14645089 14645089]
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| - | [[Category: Single protein]]
| + | |
| - | [[Category: Chandrashekar, I R.]]
| + | |
| - | [[Category: Cowsik, S M.]]
| + | |
| - | [[Category: 3 10 helix]] | + | |
| - | [[Category: dpc micelle]] | + | |
| - | [[Category: helix]] | + | |
| - | [[Category: lipid induced conformation]] | + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:26:10 2008''
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