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5jqm

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'''Unreleased structure'''
 
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The entry 5jqm is ON HOLD until Paper Publication
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==Crystal Structure of Phosphatidic acid Transporter Ups1/Mdm35 Void of Bound Phospholipid from Saccharomyces Cerevisiae at 1.5 Angstroms Resolution==
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<StructureSection load='5jqm' size='340' side='right'caption='[[5jqm]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5jqm]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JQM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JQM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jqm OCA], [https://pdbe.org/5jqm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jqm RCSB], [https://www.ebi.ac.uk/pdbsum/5jqm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jqm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MDM35_YEAST MDM35_YEAST] Involved in mitochondrial distribution and morphology. Mediates the import of UPS1, UPS2 and UPS3, 3 atypical mitochondrial intermembrane space (IMS) proteins lacking the two major IMS-targeting signals, into the intermembrane space.<ref>PMID:11907266</ref> <ref>PMID:20622808</ref> <ref>PMID:20657548</ref> [https://www.uniprot.org/uniprot/UPS1_YEAST UPS1_YEAST] Required for maintenance of normal mitochondrial morphology as well as PCP1-dependent processing of MGM1. With UPS2, controls the level of cardiolipin in mitochondria. Cardiolipin is a unique phospholipid with four fatty acid chains and is present mainly in the mitochondrial inner membrane where it stabilizes the electron transport chain supercomplex between complexes III and IV through direct interaction of their subunits.<ref>PMID:16754953</ref> <ref>PMID:19221197</ref> <ref>PMID:19506038</ref> <ref>PMID:20622808</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cardiolipin, an essential mitochondrial physiological regulator, is synthesized from phosphatidic acid (PA) in the inner mitochondrial membrane (IMM). PA is synthesized in the endoplasmic reticulum and transferred to the IMM via the outer mitochondrial membrane (OMM) under mediation by the Ups1/Mdm35 protein family. Despite the availability of numerous crystal structures, the detailed mechanism underlying PA transfer between mitochondrial membranes remains unclear. Here, a model of Ups1/Mdm35-membrane interaction is established using combined crystallographic data, all-atom molecular dynamics simulations, extensive structural comparisons, and biophysical assays. The alpha2-loop, L2-loop, and alpha3 helix of Ups1 mediate membrane interactions. Moreover, non-complexed Ups1 on membranes is found to be a key transition state for PA transfer. The membrane-bound non-complexed Ups1/ membrane-bound Ups1 ratio, which can be regulated by environmental pH, is inversely correlated with the PA transfer activity of Ups1/Mdm35. These results demonstrate a new model of the fine conformational changes of Ups1/Mdm35 during PA transfer.
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Authors: Lu, J., Chan, K.C., Zhai, Y., Fan, J., Sun, F.
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Molecular mechanism of mitochondrial phosphatidate transfer by Ups1.,Lu J, Chan C, Yu L, Fan J, Sun F, Zhai Y Commun Biol. 2020 Aug 25;3(1):468. doi: 10.1038/s42003-020-01121-x. PMID:32843686<ref>PMID:32843686</ref>
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Description: Crystal Structure of Phosphatidic acid Transporter Ups1/Mdm35 Void of Bound Phospholipid from Saccharomyces Cerevisiae at 1.5 Angstroms Resolution
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Fan, J]]
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<div class="pdbe-citations 5jqm" style="background-color:#fffaf0;"></div>
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[[Category: Zhai, Y]]
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== References ==
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[[Category: Lu, J]]
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<references/>
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[[Category: Sun, F]]
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__TOC__
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[[Category: Chan, K.C]]
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Chan KC]]
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[[Category: Fan J]]
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[[Category: Lu J]]
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[[Category: Sun F]]
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[[Category: Zhai Y]]

Current revision

Crystal Structure of Phosphatidic acid Transporter Ups1/Mdm35 Void of Bound Phospholipid from Saccharomyces Cerevisiae at 1.5 Angstroms Resolution

PDB ID 5jqm

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