5lmo

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(New page: '''Unreleased structure''' The entry 5lmo is ON HOLD Authors: Hussain, T, Llacer, J.L., Wimberly, B.T., Ramakrishnan, V Description: Structure of bacterial 30S-IF1-IF3-mRNA translation...)
Current revision (18:34, 18 October 2023) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 5lmo is ON HOLD
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==Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1B)==
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<SX load='5lmo' size='340' side='right' viewer='molstar' caption='[[5lmo]], [[Resolution|resolution]] 4.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5lmo]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LMO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LMO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lmo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lmo OCA], [https://pdbe.org/5lmo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lmo RCSB], [https://www.ebi.ac.uk/pdbsum/5lmo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lmo ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RS7_THET8 RS7_THET8] One of the primary rRNA binding proteins, it binds directly to 3'-end of the 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center. Binds mRNA and the E site tRNA blocking its exit path in the ribosome. This blockage implies that this section of the ribosome must be able to move to release the deacetylated tRNA.[HAMAP-Rule:MF_00480_B]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In bacterial translational initiation, three initiation factors (IFs 1-3) enable the selection of initiator tRNA and the start codon in the P site of the 30S ribosomal subunit. Here, we report 11 single-particle cryo-electron microscopy (cryoEM) reconstructions of the complex of bacterial 30S subunit with initiator tRNA, mRNA, and IFs 1-3, representing different steps along the initiation pathway. IF1 provides key anchoring points for IF2 and IF3, thereby enhancing their activities. IF2 positions a domain in an extended conformation appropriate for capturing the formylmethionyl moiety charged on tRNA. IF3 and tRNA undergo large conformational changes to facilitate the accommodation of the formylmethionyl-tRNA (fMet-tRNA(fMet)) into the P site for start codon recognition.
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Authors: Hussain, T, Llacer, J.L., Wimberly, B.T., Ramakrishnan, V
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Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation.,Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V Cell. 2016 Sep 22;167(1):133-144.e13. doi: 10.1016/j.cell.2016.08.074. PMID:27662086<ref>PMID:27662086</ref>
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Description: Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1B)
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Hussain, T, Llacer, J.L]]
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<div class="pdbe-citations 5lmo" style="background-color:#fffaf0;"></div>
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[[Category: Wimberly, B.T]]
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[[Category: Ramakrishnan, V]]
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==See Also==
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*[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]]
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== References ==
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<references/>
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__TOC__
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</SX>
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[[Category: Large Structures]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Hussain T]]
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[[Category: Llacer JL]]
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[[Category: Ramakrishnan V]]
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[[Category: Wimberly BT]]

Current revision

Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1B)

5lmo, resolution 4.30Å

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