5jip
From Proteopedia
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==Crystal structure of the Clostridium perfringens spore cortex lytic enzyme SleM== | ==Crystal structure of the Clostridium perfringens spore cortex lytic enzyme SleM== | ||
| - | <StructureSection load='5jip' size='340' side='right' caption='[[5jip]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='5jip' size='340' side='right'caption='[[5jip]], [[Resolution|resolution]] 1.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5jip]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JIP OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[5jip]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JIP FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jip OCA], [https://pdbe.org/5jip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jip RCSB], [https://www.ebi.ac.uk/pdbsum/5jip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jip ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/O06496_CLOPF O06496_CLOPF] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Clostridium perfringens spores employ two peptidoglycan lysins to degrade the spore cortex during germination. SleC initiates cortex hydrolysis to generate cortical fragments that are degraded further by the muramidase SleM. Here, we present the crystal structure of the C. perfringens S40 SleM protein at 1.8 A. SleM comprises an N-terminal catalytic domain that adopts an irregular alpha/beta-barrel fold that is common to GH25 family lysozymes, plus a C-terminal fibronectin type III domain. The latter is involved in forming the SleM dimer that is evident in both the crystal structure and in solution. A truncated form of SleM that lacks the FnIII domain shows reduced activity against spore sacculi indicating that this domain may have a role in facilitating the position of substrate with respect to the enzyme's active site. Proteins 2016. (c) 2016 Wiley Periodicals, Inc. | ||
| + | |||
| + | The crystal structure of Clostridium perfringens SleM, a muramidase involved in cortical hydrolysis during spore germination.,Al-Riyami B, Ustok FI, Stott K, Chirgadze DY, Christie G Proteins. 2016 Aug 2. doi: 10.1002/prot.25112. PMID:27488615<ref>PMID:27488615</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5jip" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Clostridium perfringens]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Al-Riyami B]] |
| - | [[Category: | + | [[Category: Chirgadze DY]] |
| - | [[Category: | + | [[Category: Christie G]] |
| - | [[Category: | + | [[Category: Stott K]] |
| - | [[Category: | + | [[Category: Ustok FI]] |
| - | + | ||
| - | + | ||
Current revision
Crystal structure of the Clostridium perfringens spore cortex lytic enzyme SleM
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