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| | ==Crystal structure of reductase (R) domain of non-ribosomal peptide synthetase from Mycobacterium tuberculosis== | | ==Crystal structure of reductase (R) domain of non-ribosomal peptide synthetase from Mycobacterium tuberculosis== |
| - | <StructureSection load='4dqv' size='340' side='right' caption='[[4dqv]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='4dqv' size='340' side='right'caption='[[4dqv]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4dqv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DQV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DQV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4dqv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DQV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DQV FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nrp, Rv0101, rv101 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dqv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dqv OCA], [http://pdbe.org/4dqv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dqv RCSB], [http://www.ebi.ac.uk/pdbsum/4dqv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dqv ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dqv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dqv OCA], [https://pdbe.org/4dqv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dqv RCSB], [https://www.ebi.ac.uk/pdbsum/4dqv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dqv ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/INLPA_MYCTU INLPA_MYCTU] Nonribosomal peptide synthetase (NRPS) involved in the biosynthesis of a unique class of isonitrile lipopeptides (INLPs) that seem to function as virulence factors in M.tuberculosis and to play a role in metal acquisition (PubMed:28634299). Catalyzes the final step in the pathway, i.e. the condensation of a (3R)-3-isocyanyl-fatty acyl-[ACP] to both amino groups of a lysine, producing isonitrile lipopeptides (By similarity).[UniProtKB:B2HKL9]<ref>PMID:28634299</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Haque, A S]] | + | [[Category: Large Structures]] |
| - | [[Category: Panjikar, S]] | + | [[Category: Mycobacterium tuberculosis]] |
| - | [[Category: Sankaranarayanan, R]] | + | [[Category: Haque AS]] |
| - | [[Category: Gxxgxxg motif]] | + | [[Category: Panjikar S]] |
| - | [[Category: Ligase]] | + | [[Category: Sankaranarayanan R]] |
| - | [[Category: Lipopeptide]]
| + | |
| - | [[Category: Reductase]]
| + | |
| - | [[Category: Rossmann fold]]
| + | |
| - | [[Category: Short chain dehydrogenase/reductase family]]
| + | |
| Structural highlights
Function
INLPA_MYCTU Nonribosomal peptide synthetase (NRPS) involved in the biosynthesis of a unique class of isonitrile lipopeptides (INLPs) that seem to function as virulence factors in M.tuberculosis and to play a role in metal acquisition (PubMed:28634299). Catalyzes the final step in the pathway, i.e. the condensation of a (3R)-3-isocyanyl-fatty acyl-[ACP] to both amino groups of a lysine, producing isonitrile lipopeptides (By similarity).[UniProtKB:B2HKL9][1]
Publication Abstract from PubMed
In mycobacteria, polyketide synthases and nonribosomal peptide synthetases (NRPSs) produce complex lipidic metabolites by using a thio-template mechanism of catalysis. In this study, we demonstrate that off-loading reductase (R) domain of mycobacterial NRPSs performs two consecutive [2 + 2]e(-) reductions to release thioester-bound lipopeptides as corresponding alcohols, using a nonprocessive mechanism of catalysis. The first crystal structure of an R domain from Mycobacterium tuberculosis NRPS provides strong support to this mechanistic model and suggests that the displacement of intermediate would be required for cofactor recycling. We show that 4e(-) reductases produce alcohols through a committed aldehyde intermediate, and the reduction of this intermediate is at least 10 times more efficient than the thioester-substrate. Structural and biochemical studies also provide evidence for the conformational changes associated with the reductive cycle. Further, we show that the large substrate-binding pocket with a hydrophobic platform accounts for the remarkable substrate promiscuity of these domains. Our studies present an elegant example of the recruitment of a canonical short-chain dehydrogenase/reductase family member as an off-loading domain in the context of assembly-line enzymology.
Nonprocessive [2 + 2]e- off-loading reductase domains from mycobacterial nonribosomal peptide synthetases.,Chhabra A, Haque AS, Pal RK, Goyal A, Rai R, Joshi S, Panjikar S, Pasha S, Sankaranarayanan R, Gokhale RS Proc Natl Acad Sci U S A. 2012 Apr 10;109(15):5681-6. Epub 2012 Mar 26. PMID:22451903[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Harris NC, Sato M, Herman NA, Twigg F, Cai W, Liu J, Zhu X, Downey J, Khalaf R, Martin J, Koshino H, Zhang W. Biosynthesis of isonitrile lipopeptides by conserved nonribosomal peptide synthetase gene clusters in Actinobacteria. Proc Natl Acad Sci U S A. 2017 Jul 3;114(27):7025-7030. PMID:28634299 doi:10.1073/pnas.1705016114
- ↑ Chhabra A, Haque AS, Pal RK, Goyal A, Rai R, Joshi S, Panjikar S, Pasha S, Sankaranarayanan R, Gokhale RS. Nonprocessive [2 + 2]e- off-loading reductase domains from mycobacterial nonribosomal peptide synthetases. Proc Natl Acad Sci U S A. 2012 Apr 10;109(15):5681-6. Epub 2012 Mar 26. PMID:22451903 doi:10.1073/pnas.1118680109
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