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| | ==Crystal Structure of Chlamydomonas reinhardtii Isoamylase 1 (ISA1)== | | ==Crystal Structure of Chlamydomonas reinhardtii Isoamylase 1 (ISA1)== |
| - | <StructureSection load='4j7r' size='340' side='right' caption='[[4j7r]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='4j7r' size='340' side='right'caption='[[4j7r]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4j7r]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J7R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J7R FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4j7r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J7R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J7R FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4okd|4okd]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Isa1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 CHLRE])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j7r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j7r OCA], [https://pdbe.org/4j7r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j7r RCSB], [https://www.ebi.ac.uk/pdbsum/4j7r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j7r ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isoamylase Isoamylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.68 3.2.1.68] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j7r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j7r OCA], [http://pdbe.org/4j7r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j7r RCSB], [http://www.ebi.ac.uk/pdbsum/4j7r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j7r ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q7X8Q2_CHLRE Q7X8Q2_CHLRE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chlre]] | + | [[Category: Chlamydomonas reinhardtii]] |
| - | [[Category: Isoamylase]] | + | [[Category: Large Structures]] |
| - | [[Category: Palcic, M]] | + | [[Category: Palcic M]] |
| - | [[Category: Sim, L]] | + | [[Category: Sim L]] |
| - | [[Category: Gh13 glycoside hydrolase]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
Q7X8Q2_CHLRE
Publication Abstract from PubMed
The starch debranching enzymes isoamylase 1 and 2 (ISA1 & ISA2) are known to exist in a large complex and are involved in the biosynthesis and crystallization of starch. It is suggested that the function of the complex is to remove misplaced branches of growing amylopectin molecules, which would otherwise prevent the association and crystallization of adjacent linear chains. Here, we investigate the function of ISA1 and ISA2 from starch producing alga Chlamydomonas. Through complementation studies, we confirm that the STA8 locus encodes for ISA2 and sta8 mutants lack the ISA1/ISA2 heteromeric complex. However, mutants retain a functional dimeric ISA1 that is able to partly sustain starch synthesis in vivo. To better characterize ISA1, we have overexpressed and purified ISA1 from Chlamydomonas reinhardtii (CrISA1) and solved the crystal structure to 2.3 A and in complex with maltoheptaose to 2.4 A. Analysis of the homodimeric CrISA1 structure reveals a unique elongated structure with monomers connected end-to-end. The crystal complex reveals details about the mechanism of branch binding that explains the low activity of CrISA1 towards tightly spaced branches and reveals the presence of additional secondary surface carbohydrate binding sites.
Crystal structure of the Chlamydomonas starch debranching enzyme isoamylase ISA1 reveals insights into the mechanism of branch trimming and complex assembly.,Sim L, Beeren SR, Findinier J, Dauvillee D, Ball S, Henriksen A, Palcic MM J Biol Chem. 2014 Jul 3. pii: jbc.M114.565044. PMID:24993830[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sim L, Beeren SR, Findinier J, Dauvillee D, Ball S, Henriksen A, Palcic MM. Crystal structure of the Chlamydomonas starch debranching enzyme isoamylase ISA1 reveals insights into the mechanism of branch trimming and complex assembly. J Biol Chem. 2014 Jul 3. pii: jbc.M114.565044. PMID:24993830 doi:http://dx.doi.org/10.1074/jbc.M114.565044
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