3tlj

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==Crystal structure of Trm14 from Pyrococcus furiosus in complex with S-adenosyl-L-homocysteine==
==Crystal structure of Trm14 from Pyrococcus furiosus in complex with S-adenosyl-L-homocysteine==
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<StructureSection load='3tlj' size='340' side='right' caption='[[3tlj]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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<StructureSection load='3tlj' size='340' side='right'caption='[[3tlj]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3tlj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrfu Pyrfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TLJ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3tlj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TLJ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3tm4|3tm4]], [[3tm5|3tm5]], [[3tma|3tma]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF1002 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 PYRFU])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tlj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tlj OCA], [https://pdbe.org/3tlj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tlj RCSB], [https://www.ebi.ac.uk/pdbsum/3tlj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tlj ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tlj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tlj OCA], [http://pdbe.org/3tlj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tlj RCSB], [http://www.ebi.ac.uk/pdbsum/3tlj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tlj ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/TRM14_PYRFU TRM14_PYRFU] S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the methylation of the guanosine nucleotide at position 6 (m2G6) in tRNA(Phe).<ref>PMID:22337946</ref> <ref>PMID:22362751</ref>
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Methyltransferases (MTases) form a major class of tRNA-modifying enzymes needed for the proper functioning of tRNA. Recently, RNA MTases from the TrmN/Trm14 family that are present in Archaea, Bacteria and Eukaryota have been shown to specifically modify tRNA(Phe) at guanosine 6 in the tRNA acceptor stem. Here, we report the first X-ray crystal structures of the tRNA m(2)G6 (N(2)-methylguanosine) MTase (TTC)TrmN from Thermus thermophilus and its ortholog (Pf)Trm14 from Pyrococcus furiosus. Structures of (Pf)Trm14 were solved in complex with the methyl donor S-adenosyl-l-methionine (SAM or AdoMet), as well as the reaction product S-adenosyl-homocysteine (SAH or AdoHcy) and the inhibitor sinefungin. (TTC)TrmN and (Pf)Trm14 consist of an N-terminal THUMP domain fused to a catalytic Rossmann-fold MTase (RFM) domain. These results represent the first crystallographic structure analysis of proteins containing both THUMP and RFM domain, and hence provide further insight in the contribution of the THUMP domain in tRNA recognition and catalysis. Electrostatics and conservation calculations suggest a main tRNA binding surface in a groove between the THUMP domain and the MTase domain. This is further supported by a docking model of TrmN in complex with tRNA(Phe) of T. thermophilus and via site-directed mutagenesis.
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Crystal structures of the tRNA:m2G6 methyltransferase Trm14/TrmN from two domains of life.,Fislage M, Roovers M, Tuszynska I, Bujnicki JM, Droogmans L, Versees W Nucleic Acids Res. 2012 Feb 22. PMID:22362751<ref>PMID:22362751</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3tlj" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[TRNA methyltransferase|TRNA methyltransferase]]
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*[[TRNA methyltransferase 3D structures|TRNA methyltransferase 3D structures]]
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*[[TRNA:m2G6 methyltransferase TrmN/Trm14|TRNA:m2G6 methyltransferase TrmN/Trm14]]
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Pyrfu]]
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[[Category: Large Structures]]
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[[Category: Bujnicki, J M]]
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[[Category: Pyrococcus furiosus DSM 3638]]
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[[Category: Droogmans, L]]
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[[Category: Bujnicki JM]]
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[[Category: Fislage, M]]
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[[Category: Droogmans L]]
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[[Category: Roovers, M]]
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[[Category: Fislage M]]
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[[Category: Tuszynska, I]]
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[[Category: Roovers M]]
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[[Category: Versees, W]]
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[[Category: Tuszynska I]]
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[[Category: Methyltransferase]]
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[[Category: Versees W]]
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[[Category: Rossmann fold]]
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[[Category: Thump domain]]
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[[Category: Transferase]]
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[[Category: Trna methyltransferase]]
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Current revision

Crystal structure of Trm14 from Pyrococcus furiosus in complex with S-adenosyl-L-homocysteine

PDB ID 3tlj

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