4en5

<StructureSection load='4en5' size='340' side='right' caption='[[4en5]], [[Resolution|resolution]] 2.96&Aring;' scene=''>

<table><tr><td colspan='2'>[[4en5]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EN5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EN5 FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F:FLUORIDE+ION'>F</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TL:THALLIUM+(I)+ION'>TL</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ena|4ena]], [[4enb|4enb]], [[4enc|4enc]], [[3vrs|3vrs]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4en5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4en5 OCA], [http://pdbe.org/4en5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4en5 RCSB], [http://www.ebi.ac.uk/pdbsum/4en5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4en5 ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Significant advances in our understanding of RNA architecture, folding and recognition have emerged from structure-function studies on riboswitches, non-coding RNAs whose sensing domains bind small ligands and whose adjacent expression platforms contain RNA elements involved in the control of gene regulation. We now report on the ligand-bound structure of the Thermotoga petrophila fluoride riboswitch, which adopts a higher-order RNA architecture stabilized by pseudoknot and long-range reversed Watson-Crick and Hoogsteen A*U pair formation. The bound fluoride ion is encapsulated within the junctional architecture, anchored in place through direct coordination to three Mg(2+) ions, which in turn are octahedrally coordinated to water molecules and five inwardly pointing backbone phosphates. Our structure of the fluoride riboswitch in the bound state shows how RNA can form a binding pocket selective for fluoride, while discriminating against larger halide ions. The T. petrophila fluoride riboswitch probably functions in gene regulation through a transcription termination mechanism.

Fluoride ion encapsulation by Mg2+ ions and phosphates in a fluoride riboswitch.,Ren A, Rajashankar KR, Patel DJ Nature. 2012 May 13;486(7401):85-9. doi: 10.1038/nature11152. PMID:22678284<ref>PMID:22678284</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 4en5" style="background-color:#fffaf0;"></div>

*[[Riboswitch|Riboswitch]]

[[Category: Patel, D J]]

[[Category: Rajashankar, K R]]

[[Category: Ren, A M]]

[[Category: Pseudoknot]]

[[Category: Rna]]