4jix

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==Crystal structure of the metallopeptidase zymogen of Methanocaldococcus jannaschii jannalysin==
==Crystal structure of the metallopeptidase zymogen of Methanocaldococcus jannaschii jannalysin==
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<StructureSection load='4jix' size='340' side='right' caption='[[4jix]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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<StructureSection load='4jix' size='340' side='right'caption='[[4jix]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4jix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Metja Metja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JIX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JIX FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4jix]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii_DSM_2661 Methanocaldococcus jannaschii DSM 2661]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JIX FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4jiu|4jiu]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MJ0123 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jix OCA], [https://pdbe.org/4jix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jix RCSB], [https://www.ebi.ac.uk/pdbsum/4jix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jix ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jix OCA], [http://pdbe.org/4jix PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4jix RCSB], [http://www.ebi.ac.uk/pdbsum/4jix PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4jix ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Y123_METJA Y123_METJA]
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In the search for structural models of integral-membrane metallopeptidases (MPs), we discovered three related proteins from thermophilic prokaryotes, which we grouped into a novel family called "minigluzincins." We determined the crystal structures of the zymogens of two of these (Pyrococcus abyssi proabylysin and Methanocaldococcus jannaschii projannalysin), which are soluble and, with approximately 100 residues, constitute the shortest structurally characterized MPs to date. Despite relevant sequence and structural similarity, the structures revealed two unique mechanisms of latency maintenance through the C-terminal segments previously unseen in MPs as follows: intramolecular, through an extended tail, in proabylysin, and crosswise intermolecular, through a helix swap, in projannalysin. In addition, structural and sequence comparisons revealed large similarity with MPs of the gluzincin tribe such as thermolysin, leukotriene A4 hydrolase relatives, and cowrins. Noteworthy, gluzincins mostly contain a glutamate as third characteristic zinc ligand, whereas minigluzincins have a histidine. Sequence and structural similarity further allowed us to ascertain that minigluzincins are very similar to the catalytic domains of integral membrane MPs of the MEROPS database families M48 and M56, such as FACE1, HtpX, Oma1, and BlaR1/MecR1, which are provided with trans-membrane helices flanking or inserted into a minigluzincin-like catalytic domain. In a time where structural biochemistry of integral-membrane proteins in general still faces formidable challenges, the minigluzincin soluble minimal scaffold may contribute to our understanding of the working mechanisms of these membrane MPs and to the design of novel inhibitors through structure-aided rational drug design approaches.
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A novel family of soluble minimal scaffolds provides structural insight into the catalytic domains of integral membrane metallopeptidases.,Lopez-Pelegrin M, Cerda-Costa N, Martinez-Jimenez F, Cintas-Pedrola A, Canals A, Peinado JR, Marti-Renom MA, Lopez-Otin C, Arolas JL, Gomis-Ruth FX J Biol Chem. 2013 Jul 19;288(29):21279-94. doi: 10.1074/jbc.M113.476580. Epub, 2013 Jun 3. PMID:23733187<ref>PMID:23733187</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4jix" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Metja]]
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[[Category: Large Structures]]
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[[Category: Arolas, J L]]
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[[Category: Methanocaldococcus jannaschii DSM 2661]]
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[[Category: Canals, A]]
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[[Category: Arolas JL]]
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[[Category: Cerda-Costa, N]]
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[[Category: Canals A]]
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[[Category: Cintas-Pedrola, A]]
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[[Category: Cerda-Costa N]]
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[[Category: Gomis-Ruth, F X]]
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[[Category: Cintas-Pedrola A]]
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[[Category: Lopez-Otin, C]]
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[[Category: Gomis-Ruth FX]]
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[[Category: Lopez-Pelegrin, M]]
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[[Category: Lopez-Otin C]]
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[[Category: Marti-Renom, M A]]
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[[Category: Lopez-Pelegrin M]]
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[[Category: Martinez-Jimenez, F]]
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[[Category: Marti-Renom MA]]
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[[Category: Peinado, J R]]
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[[Category: Martinez-Jimenez F]]
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[[Category: Hydrolase]]
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[[Category: Peinado JR]]
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[[Category: Metallopeptidase zymogen]]
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[[Category: Minigluzincin]]
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Current revision

Crystal structure of the metallopeptidase zymogen of Methanocaldococcus jannaschii jannalysin

PDB ID 4jix

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