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| | ==Crystal Structure Staphylococcus aureus ESSB cytoplasmic fragment== | | ==Crystal Structure Staphylococcus aureus ESSB cytoplasmic fragment== |
| - | <StructureSection load='4ann' size='340' side='right' caption='[[4ann]], [[Resolution|resolution]] 1.05Å' scene=''> | + | <StructureSection load='4ann' size='340' side='right'caption='[[4ann]], [[Resolution|resolution]] 1.05Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ann]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staa8 Staa8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ANN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ANN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ann]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ANN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ANN FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ano|4ano]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.05Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ann FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ann OCA], [http://pdbe.org/4ann PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ann RCSB], [http://www.ebi.ac.uk/pdbsum/4ann PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ann ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ann FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ann OCA], [https://pdbe.org/4ann PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ann RCSB], [https://www.ebi.ac.uk/pdbsum/4ann PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ann ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/ESSB_STAA8 ESSB_STAA8] Component of the type VII secretion system (Ess) (Probable). Required for the secretion of EsxA and EsxB (By similarity).[UniProtKB:P0C053] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Staa8]] | + | [[Category: Large Structures]] |
| - | [[Category: Fyfe, P K]] | + | [[Category: Staphylococcus aureus subsp. aureus NCTC 8325]] |
| - | [[Category: Hunter, W N]] | + | [[Category: Fyfe PK]] |
| - | [[Category: Palmer, T]] | + | [[Category: Hunter WN]] |
| - | [[Category: Zoltner, M]] | + | [[Category: Palmer T]] |
| - | [[Category: Ess type vii secretion system]] | + | [[Category: Zoltner M]] |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Membrane secretion]]
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| - | [[Category: Virulence]]
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| Structural highlights
Function
ESSB_STAA8 Component of the type VII secretion system (Ess) (Probable). Required for the secretion of EsxA and EsxB (By similarity).[UniProtKB:P0C053]
Publication Abstract from PubMed
The type VII protein translocation/secretion system, unique to Gram-positive bacteria is a key virulence determinant in Staphylococcus aureus. We aim to characterise the architecture of this secretion machinery and now describe a study of S. aureus EssB, a 52-kDa bitopic membrane protein essential for secretion of the ESAT-6-family-proteins, the prototypic substrate of Type VII secretion. Full-length EssB was heterologously expressed in Escherichia coli, solubilised from the bacterial membrane, purified to homogeneity and shown to be dimeric. A C-terminal truncation, EssBC, and two soluble fragments termed EssB-N and EssB-C, predicted to occur on either side of the cytoplasmic membrane, have been successfully purified in recombinant form, characterised and together with the full-length protein used in crystallisation trials. EssB-N, the 25 kDa N-terminal cytoplasmic fragment, gave well-ordered crystals and we report the structure, determined by single-wavelength anomalous diffraction (SAD) targeting an SeMet derivative, refined to atomic (1.05 A) resolution. EssB-N is dimeric in solution but crystallises as a monomer and displays a fold composed of two globular domains separated by a cleft. The structure is related to that of Ser/Thr protein kinases and our study identifies that the type VII secretion system exploits and re-uses a stable modular entity and fold that has evolved to participate in protein-protein interactions in a similar fashion to the catalytically inert pseudokinases.
Characterisation of Staphylococcus aureus EssB, an integral membrane component of the Type VII secretion system; atomic resolution crystal structure of the cytoplasmic segment.,Zoltner M, Fyfe PK, Palmer T, Hunter WN Biochem J. 2012 Oct 25. PMID:23098276[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zoltner M, Fyfe PK, Palmer T, Hunter WN. Characterisation of Staphylococcus aureus EssB, an integral membrane component of the Type VII secretion system; atomic resolution crystal structure of the cytoplasmic segment. Biochem J. 2012 Oct 25. PMID:23098276 doi:http://dx.doi.org/10.1042/BJ20121209
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