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| - | ==STRUCTURE OF DUCK RIG-I C-TERMINAL DOMAIN (CTD) WITH 14-MER DSRNA== | + | ==Structure of duck RIG-I C-terminal domain (CTD) with 14-mer dSRNA== |
| - | <StructureSection load='4a2x' size='340' side='right' caption='[[4a2x]], [[Resolution|resolution]] 4.00Å' scene=''> | + | <StructureSection load='4a2x' size='340' side='right'caption='[[4a2x]], [[Resolution|resolution]] 4.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4a2x]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A2X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A2X FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4a2x]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A2X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A2X FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4a2p|4a2p]], [[4a2v|4a2v]], [[4a36|4a36]], [[4a2w|4a2w]], [[4a2q|4a2q]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a2x OCA], [http://pdbe.org/4a2x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4a2x RCSB], [http://www.ebi.ac.uk/pdbsum/4a2x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4a2x ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a2x OCA], [https://pdbe.org/4a2x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a2x RCSB], [https://www.ebi.ac.uk/pdbsum/4a2x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a2x ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/D3TI84_ANAPL D3TI84_ANAPL] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Anapl]] | + | [[Category: Anas platyrhynchos]] |
| - | [[Category: Cusack, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Kowalinski, E]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Lunardi, T]] | + | [[Category: Cusack S]] |
| - | [[Category: Mccarthy, A A]] | + | [[Category: Kowalinski E]] |
| - | [[Category: Antiviral signalling pathway]] | + | [[Category: Lunardi T]] |
| - | [[Category: Atp and dsrna binding]]
| + | [[Category: McCarthy AA]] |
| - | [[Category: Rna binding protein-rna complex]]
| + | |
| - | [[Category: Superfamily 2 rna helicase]] | + | |
| Structural highlights
Function
D3TI84_ANAPL
Publication Abstract from PubMed
RIG-I is a key innate immune pattern-recognition receptor that triggers interferon expression upon detection of intracellular 5'triphosphate double-stranded RNA (5'ppp-dsRNA) of viral origin. RIG-I comprises N-terminal caspase activation and recruitment domains (CARDs), a DECH helicase, and a C-terminal domain (CTD). We present crystal structures of the ligand-free, autorepressed, and RNA-bound, activated states of RIG-I. Inactive RIG-I has an open conformation with the CARDs sequestered by a helical domain inserted between the two helicase moieties. ATP and dsRNA binding induce a major rearrangement to a closed conformation in which the helicase and CTD bind the blunt end 5'ppp-dsRNA with perfect complementarity but incompatibly with continued CARD binding. We propose that after initial binding of 5'ppp-dsRNA to the flexibly linked CTD, co-operative tight binding of ATP and RNA to the helicase domain liberates the CARDs for downstream signaling. These findings significantly advance our molecular understanding of the activation of innate immune signaling helicases.
Structural Basis for the Activation of Innate Immune Pattern-Recognition Receptor RIG-I by Viral RNA.,Kowalinski E, Lunardi T, McCarthy AA, Louber J, Brunel J, Grigorov B, Gerlier D, Cusack S Cell. 2011 Oct 14;147(2):423-35. PMID:22000019[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kowalinski E, Lunardi T, McCarthy AA, Louber J, Brunel J, Grigorov B, Gerlier D, Cusack S. Structural Basis for the Activation of Innate Immune Pattern-Recognition Receptor RIG-I by Viral RNA. Cell. 2011 Oct 14;147(2):423-35. PMID:22000019 doi:10.1016/j.cell.2011.09.039
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