|
|
| (5 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Structure of linear gramicidin D obtained using Type I crystals grown in a lipid cubic phase.== | | ==Structure of linear gramicidin D obtained using Type I crystals grown in a lipid cubic phase.== |
| - | <StructureSection load='2xdc' size='340' side='right' caption='[[2xdc]], [[Resolution|resolution]] 1.70Å' scene=''> | + | <StructureSection load='2xdc' size='340' side='right'caption='[[2xdc]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2xdc]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Brevibacillus_brevis Brevibacillus brevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XDC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XDC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2xdc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevibacillus_brevis Brevibacillus brevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XDC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XDC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DLE:D-LEUCINE'>DLE</scene>, <scene name='pdbligand=DVA:D-VALINE'>DVA</scene>, <scene name='pdbligand=ETA:ETHANOLAMINE'>ETA</scene>, <scene name='pdbligand=FVA:N-FORMYL-L-VALINE'>FVA</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=DLE:D-LEUCINE'>DLE</scene>, <scene name='pdbligand=DVA:D-VALINE'>DVA</scene>, <scene name='pdbligand=ETA:ETHANOLAMINE'>ETA</scene>, <scene name='pdbligand=FVA:N-FORMYL-L-VALINE'>FVA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PRD_000150:GRAMICIDIN+A'>PRD_000150</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1tk2|1tk2]], [[1av2|1av2]], [[1bdw|1bdw]], [[1c4d|1c4d]], [[1gmk|1gmk]], [[1grm|1grm]], [[1jno|1jno]], [[1kqe|1kqe]], [[1mag|1mag]], [[1mic|1mic]], [[1ng8|1ng8]], [[1nrm|1nrm]], [[1nru|1nru]], [[1nt5|1nt5]], [[1jo3|1jo3]], [[1jo4|1jo4]], [[1nt6|1nt6]], [[1tkq|1tkq]], [[1w5u|1w5u]], [[2izq|2izq]], [[3l8l|3l8l]], [[1al4|1al4]], [[1alx|1alx]], [[1alz|1alz]]</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xdc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xdc OCA], [https://pdbe.org/2xdc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xdc RCSB], [https://www.ebi.ac.uk/pdbsum/2xdc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xdc ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xdc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xdc OCA], [http://pdbe.org/2xdc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xdc RCSB], [http://www.ebi.ac.uk/pdbsum/2xdc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xdc ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| Line 26: |
Line 25: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Brevibacillus brevis]] | | [[Category: Brevibacillus brevis]] |
| - | [[Category: Aragao, D]] | + | [[Category: Large Structures]] |
| - | [[Category: Caffrey, M]] | + | [[Category: Aragao D]] |
| - | [[Category: Hoefer, N]] | + | [[Category: Caffrey M]] |
| - | [[Category: Antibacterial]] | + | [[Category: Hoefer N]] |
| - | [[Category: Antibiotic]]
| + | |
| - | [[Category: Antifungal]]
| + | |
| - | [[Category: Bilayer]]
| + | |
| - | [[Category: Ion channel]]
| + | |
| - | [[Category: Lipid cubic phase]]
| + | |
| - | [[Category: Mesophase sponge phase]]
| + | |
| - | [[Category: Monoolein]]
| + | |
| Structural highlights
2xdc is a 6 chain structure with sequence from Brevibacillus brevis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 1.7Å |
| Ligands: | , , , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Publication Abstract from PubMed
Structure determination of membrane proteins by crystallographic means has been facilitated by crystallization in lipidic mesophases. It has been suggested, however, that this so-called in meso method, as originally implemented, would not apply to small protein targets having </=4 transmembrane crossings. In our study, the hypothesis that the inherent flexibility of the mesophase would enable crystallogenesis of small proteins was tested using a transmembrane pentadecapeptide, linear gramicidin, which produced structure-grade crystals. This result suggests that the in meso method should be considered as a viable means for high-resolution structure determination of integral membrane peptides, many of which are predicted to be coded for in the human genome.
Crystallizing transmembrane peptides in lipidic mesophases.,Hofer N, Aragao D, Caffrey M Biophys J. 2010 Aug 4;99(3):L23-5. PMID:20682243[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Hofer N, Aragao D, Caffrey M. Crystallizing transmembrane peptides in lipidic mesophases. Biophys J. 2010 Aug 4;99(3):L23-5. PMID:20682243 doi:10.1016/j.bpj.2010.05.011
|
