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1nhw

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[[Image:1nhw.gif|left|200px]]
 
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{{Structure
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==Crystal Structure Analysis of Plasmodium falciparum enoyl-acyl-carrier-protein reductase==
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|PDB= 1nhw |SIZE=350|CAPTION= <scene name='initialview01'>1nhw</scene>, resolution 2.35&Aring;
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<StructureSection load='1nhw' size='340' side='right'caption='[[1nhw]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=TCC:2-(2,4-DICHLORO-PHENYLAMINO)-PHENOL'>TCC</scene>
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<table><tr><td colspan='2'>[[1nhw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NHW FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=TCC:2-(2,4-DICHLORO-PHENYLAMINO)-PHENOL'>TCC</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nhw OCA], [https://pdbe.org/1nhw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nhw RCSB], [https://www.ebi.ac.uk/pdbsum/1nhw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nhw ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1nhd|1NHD]], [[1nhg|1NHG]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nhw OCA], [http://www.ebi.ac.uk/pdbsum/1nhw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nhw RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Q9BH77_PLAFA Q9BH77_PLAFA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nh/1nhw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nhw ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure Analysis of Plasmodium falciparum enoyl-acyl-carrier-protein reductase'''
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==See Also==
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*[[Enoyl-Acyl-Carrier Protein Reductase 3D structures|Enoyl-Acyl-Carrier Protein Reductase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The human malaria parasite Plasmodium falciparum synthesizes fatty acids using a type II pathway that is absent in humans. The final step in fatty acid elongation is catalyzed by enoyl acyl carrier protein reductase, a validated antimicrobial drug target. Here, we report the cloning and expression of the P. falciparum enoyl acyl carrier protein reductase gene, which encodes a 50-kDa protein (PfENR) predicted to target to the unique parasite apicoplast. Purified PfENR was crystallized, and its structure resolved as a binary complex with NADH, a ternary complex with triclosan and NAD(+), and as ternary complexes bound to the triclosan analogs 1 and 2 with NADH. Novel structural features were identified in the PfENR binding loop region that most closely resembled bacterial homologs; elsewhere the protein was similar to ENR from the plant Brassica napus (root mean square for Calphas, 0.30 A). Triclosan and its analogs 1 and 2 killed multidrug-resistant strains of intra-erythrocytic P. falciparum parasites at sub to low micromolar concentrations in vitro. These data define the structural basis of triclosan binding to PfENR and will facilitate structure-based optimization of PfENR inhibitors.
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[[Category: Large Structures]]
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==About this Structure==
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1NHW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NHW OCA].
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==Reference==
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Structural elucidation of the specificity of the antibacterial agent triclosan for malarial enoyl acyl carrier protein reductase., Perozzo R, Kuo M, Sidhu AS, Valiyaveettil JT, Bittman R, Jacobs WR Jr, Fidock DA, Sacchettini JC, J Biol Chem. 2002 Apr 12;277(15):13106-14. Epub 2002 Jan 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11792710 11792710]
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[[Category: Enoyl-[acyl-carrier-protein] reductase (NADH)]]
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[[Category: Plasmodium falciparum]]
[[Category: Plasmodium falciparum]]
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[[Category: Protein complex]]
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[[Category: Bittman R]]
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[[Category: Bittman, R.]]
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[[Category: Fidock DA]]
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[[Category: Fidock, D A.]]
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[[Category: Jacobs Jr WR]]
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[[Category: Jr., W R.Jacobs.]]
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[[Category: Kuo M]]
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[[Category: Kuo, M.]]
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[[Category: Perozzo R]]
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[[Category: Perozzo, R.]]
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[[Category: Sacchettini JC]]
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[[Category: Sacchettini, J C.]]
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[[Category: Sidhu AS]]
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[[Category: Sidhu, A S.]]
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[[Category: Valiyaveettil JT]]
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[[Category: Valiyaveettil, J T.]]
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[[Category: nadh]]
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[[Category: rossmann fold]]
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[[Category: short chain dehydrogenase reductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:30:38 2008''
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Current revision

Crystal Structure Analysis of Plasmodium falciparum enoyl-acyl-carrier-protein reductase

PDB ID 1nhw

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