1njn

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[[Image:1njn.gif|left|200px]]
 
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{{Structure
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==The crystal structure of the 50S Large ribosomal subunit from Deinococcus radiodurans complexed with the antibiotic sparsomycin==
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|PDB= 1njn |SIZE=350|CAPTION= <scene name='initialview01'>1njn</scene>, resolution 3.70&Aring;
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<StructureSection load='1njn' size='340' side='right'caption='[[1njn]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=SPS:SPARSOMYCIN'>SPS</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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<table><tr><td colspan='2'>[[1njn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NJN FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.7&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SPS:SPARSOMYCIN'>SPS</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1njn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1njn OCA], [https://pdbe.org/1njn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1njn RCSB], [https://www.ebi.ac.uk/pdbsum/1njn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1njn ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1njm|1NJM]], [[1njo|1NJO]], [[1njp|1NJP]], [[1k01|1K01]], [[1jzx|1JZX]], [[1jzy|1JZY]], [[1jzz|1JZZ]], [[1k00|1K00]], [[1nkw|1NKW]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1njn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1njn OCA], [http://www.ebi.ac.uk/pdbsum/1njn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1njn RCSB]</span>
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<div style="background-color:#fffaf0;">
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}}
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== Publication Abstract from PubMed ==
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'''The crystal structure of the 50S Large ribosomal subunit from Deinococcus radiodurans complexed with the antibiotic sparsomycin'''
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==Overview==
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Crystal structures of tRNA mimics complexed with the large ribosomal subunit of Deinococcus radiodurans indicate that remote interactions determine the precise orientation of tRNA in the peptidyl-transferase center (PTC). The PTC tolerates various orientations of puromycin derivatives and its flexibility allows the conformational rearrangements required for peptide-bond formation. Sparsomycin binds to A2602 and alters the PTC conformation. H69, the intersubunit-bridge connecting the PTC and decoding site, may also participate in tRNA placement and translocation. A spiral rotation of the 3' end of the A-site tRNA around a 2-fold axis of symmetry identified within the PTC suggests a unified ribosomal machinery for peptide-bond formation, A-to-P-site translocation, and entrance of nascent proteins into the exit tunnel. Similar 2-fold related regions, detected in all known structures of large ribosomal subunits, indicate the universality of this mechanism.
Crystal structures of tRNA mimics complexed with the large ribosomal subunit of Deinococcus radiodurans indicate that remote interactions determine the precise orientation of tRNA in the peptidyl-transferase center (PTC). The PTC tolerates various orientations of puromycin derivatives and its flexibility allows the conformational rearrangements required for peptide-bond formation. Sparsomycin binds to A2602 and alters the PTC conformation. H69, the intersubunit-bridge connecting the PTC and decoding site, may also participate in tRNA placement and translocation. A spiral rotation of the 3' end of the A-site tRNA around a 2-fold axis of symmetry identified within the PTC suggests a unified ribosomal machinery for peptide-bond formation, A-to-P-site translocation, and entrance of nascent proteins into the exit tunnel. Similar 2-fold related regions, detected in all known structures of large ribosomal subunits, indicate the universality of this mechanism.
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==About this Structure==
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Structural basis of the ribosomal machinery for peptide bond formation, translocation, and nascent chain progression.,Bashan A, Agmon I, Zarivach R, Schluenzen F, Harms J, Berisio R, Bartels H, Franceschi F, Auerbach T, Hansen HA, Kossoy E, Kessler M, Yonath A Mol Cell. 2003 Jan;11(1):91-102. PMID:12535524<ref>PMID:12535524</ref>
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1NJN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJN OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural basis of the ribosomal machinery for peptide bond formation, translocation, and nascent chain progression., Bashan A, Agmon I, Zarivach R, Schluenzen F, Harms J, Berisio R, Bartels H, Franceschi F, Auerbach T, Hansen HA, Kossoy E, Kessler M, Yonath A, Mol Cell. 2003 Jan;11(1):91-102. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12535524 12535524]
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</div>
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<div class="pdbe-citations 1njn" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Deinococcus radiodurans]]
[[Category: Deinococcus radiodurans]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Agmon, I.]]
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[[Category: Agmon I]]
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[[Category: Bartels, H.]]
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[[Category: Bartels H]]
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[[Category: Bashan, A.]]
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[[Category: Bashan A]]
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[[Category: Berisio, R.]]
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[[Category: Berisio R]]
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[[Category: Hansen, H A.]]
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[[Category: Hansen HA]]
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[[Category: Harms, J M.]]
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[[Category: Harms JM]]
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[[Category: Schluenzen, F.]]
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[[Category: Schluenzen F]]
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[[Category: Yonath, A.]]
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[[Category: Yonath A]]
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[[Category: Zarivatch, R.]]
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[[Category: Zarivatch R]]
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[[Category: peptide bond formation]]
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[[Category: peptidyl-transferase]]
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[[Category: puromycin]]
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[[Category: ribosome]]
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[[Category: sparsomycin]]
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[[Category: trna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:31:16 2008''
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Current revision

The crystal structure of the 50S Large ribosomal subunit from Deinococcus radiodurans complexed with the antibiotic sparsomycin

PDB ID 1njn

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