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| | ==Crystal structure of Kluyveromyces polysporus Argonaute with a guide RNA== | | ==Crystal structure of Kluyveromyces polysporus Argonaute with a guide RNA== |
| - | <StructureSection load='4f1n' size='340' side='right' caption='[[4f1n]], [[Resolution|resolution]] 3.19Å' scene=''> | + | <StructureSection load='4f1n' size='340' side='right'caption='[[4f1n]], [[Resolution|resolution]] 3.19Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4f1n]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Kluyveromyces_polysporus Kluyveromyces polysporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F1N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F1N FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4f1n]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Vanderwaltozyma_polyspora_DSM_70294 Vanderwaltozyma polyspora DSM 70294]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F1N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F1N FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Kpol_520p25 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=436907 Kluyveromyces polysporus])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.187Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f1n OCA], [http://pdbe.org/4f1n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f1n RCSB], [http://www.ebi.ac.uk/pdbsum/4f1n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f1n ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f1n OCA], [https://pdbe.org/4f1n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f1n RCSB], [https://www.ebi.ac.uk/pdbsum/4f1n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f1n ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A7TMA9_VANPO A7TMA9_VANPO] |
| - | The RNA-induced silencing complex, comprising Argonaute and guide RNA, mediates RNA interference. Here we report the 3.2 A crystal structure of Kluyveromyces polysporus Argonaute (KpAGO) fortuitously complexed with guide RNA originating from small-RNA duplexes autonomously loaded by recombinant KpAGO. Despite their diverse sequences, guide-RNA nucleotides 1-8 are positioned similarly, with sequence-independent contacts to bases, phosphates and 2'-hydroxyl groups pre-organizing the backbone of nucleotides 2-8 in a near-A-form conformation. Compared with prokaryotic Argonautes, KpAGO has numerous surface-exposed insertion segments, with a cluster of conserved insertions repositioning the N domain to enable full propagation of guide-target pairing. Compared with Argonautes in inactive conformations, KpAGO has a hydrogen-bond network that stabilizes an expanded and repositioned loop, which inserts an invariant glutamate into the catalytic pocket. Mutation analyses and analogies to ribonuclease H indicate that insertion of this glutamate finger completes a universally conserved catalytic tetrad, thereby activating Argonaute for RNA cleavage.
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| - | Structure of yeast Argonaute with guide RNA.,Nakanishi K, Weinberg DE, Bartel DP, Patel DJ Nature. 2012 Jun 20;486(7403):368-74. doi: 10.1038/nature11211. PMID:22722195<ref>PMID:22722195</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
| + | |
| - | <div class="pdbe-citations 4f1n" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Kluyveromyces polysporus]] | + | [[Category: Large Structures]] |
| - | [[Category: Bartel, D P]] | + | [[Category: Vanderwaltozyma polyspora DSM 70294]] |
| - | [[Category: Nakanishi, K]] | + | [[Category: Bartel DP]] |
| - | [[Category: Patel, D J]] | + | [[Category: Nakanishi K]] |
| - | [[Category: Weinberg, D E]] | + | [[Category: Patel DJ]] |
| - | [[Category: Argonaute]] | + | [[Category: Weinberg DE]] |
| - | [[Category: Rna binding protein-rna complex]]
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| - | [[Category: Rnai]]
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| - | [[Category: Rnase h]]
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