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| | ==CCAAT-binding complex from Aspergillus nidulans== | | ==CCAAT-binding complex from Aspergillus nidulans== |
| - | <StructureSection load='4g91' size='340' side='right' caption='[[4g91]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='4g91' size='340' side='right'caption='[[4g91]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4g91]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/A._nidulans A. nidulans] and [http://en.wikipedia.org/wiki/Aspergillus_nidulans Aspergillus nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G91 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G91 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4g91]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans Aspergillus nidulans] and [https://en.wikipedia.org/wiki/Aspergillus_nidulans_FGSC_A4 Aspergillus nidulans FGSC A4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G91 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G91 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1n1j|1n1j]], [[4g92|4g92]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hapB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=162425 A. nidulans]), AN4034.2, ANIA_04034 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227321 Aspergillus nidulans]), AN6492.2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227321 Aspergillus nidulans])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g91 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g91 OCA], [https://pdbe.org/4g91 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g91 RCSB], [https://www.ebi.ac.uk/pdbsum/4g91 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g91 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g91 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g91 OCA], [http://pdbe.org/4g91 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4g91 RCSB], [http://www.ebi.ac.uk/pdbsum/4g91 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4g91 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/P87249_EMEND P87249_EMEND] |
| - | The CCAAT box is a frequent element of eukaryotic promoters, and its specific recognition by the conserved heterotrimeric CCAAT-binding complex (CBC) constitutes a key step in promoter organization and regulation of transcription. Here, we report the crystal structures of the CBC from Aspergillus nidulans in the absence and in complex with double-stranded DNA at 1.8 A resolution. The histone-like subunits HapC and HapE induce nucleosome-like DNA bending by interacting with the sugar-phosphate backbone. Minor groove sensing and widening by subunit HapB tightly anchor the CBC to the CCAAT box, as shown by structural and biochemical data. Furthermore, crucial interactions of the DNA duplex with subunit HapB provide an explanation for the sequence specificity of the CBC. The herein-described mode of transcription factor binding answers the question of how histone proteins gained sequence specificity for the CCAAT box.
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| - | DNA minor groove sensing and widening by the CCAAT-binding complex.,Huber EM, Scharf DH, Hortschansky P, Groll M, Brakhage AA Structure. 2012 Oct 10;20(10):1757-68. doi: 10.1016/j.str.2012.07.012. Epub 2012 , Aug 16. PMID:22902862<ref>PMID:22902862</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
| + | |
| - | <div class="pdbe-citations 4g91" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: A. nidulans]] | |
| | [[Category: Aspergillus nidulans]] | | [[Category: Aspergillus nidulans]] |
| - | [[Category: Brakhage, A A]] | + | [[Category: Aspergillus nidulans FGSC A4]] |
| - | [[Category: Groll, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Hortschansky, P]] | + | [[Category: Brakhage AA]] |
| - | [[Category: Huber, E M]] | + | [[Category: Groll M]] |
| - | [[Category: Scharf, D H]] | + | [[Category: Hortschansky P]] |
| - | [[Category: Ccaat-binding complex]] | + | [[Category: Huber EM]] |
| - | [[Category: Histone fold motif]] | + | [[Category: Scharf DH]] |
| - | [[Category: Minor groove binding]]
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| - | [[Category: Nucleosome]]
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| - | [[Category: Nucleus]]
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| - | [[Category: Specific binding to the ccaat-box]]
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| - | [[Category: Transcription]]
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| - | [[Category: Transcription factor]]
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