3ady

<StructureSection load='3ady' size='340' side='right' caption='[[3ady]], [[Resolution|resolution]] 2.00&Aring;' scene=''>

<table><tr><td colspan='2'>[[3ady]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legph Legph]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ADY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ADY FirstGlance]. <br>

</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dotD, lpg2674 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ady FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ady OCA], [http://pdbe.org/3ady PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ady RCSB], [http://www.ebi.ac.uk/pdbsum/3ady PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ady ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

The Dot/Icm type IVB secretion system (T4BSS) is a pivotal determinant of Legionella pneumophila pathogenesis. L. pneumophila translocate more than 100 effector proteins into host cytoplasm using Dot/Icm T4BSS, modulating host cellular functions to establish a replicative niche within host cells. The T4BSS core complex spanning the inner and outer membranes is thought to be made up of at least five proteins: DotC, DotD, DotF, DotG and DotH. DotH is the outer membrane protein; its targeting depends on lipoproteins DotC and DotD. However, the core complex structure and assembly mechanism are still unknown. Here, we report the crystal structure of DotD at 2.0 A resolution. The structure of DotD is distinct from that of VirB7, the outer membrane lipoprotein of the type IVA secretion system. In contrast, the C-terminal domain of DotD is remarkably similar to the N-terminal subdomain of secretins, the integral outer membrane proteins that form substrate conduits for the type II and the type III secretion systems (T2SS and T3SS). A short beta-segment in the otherwise disordered N-terminal region, located on the hydrophobic cleft of the C-terminal domain, is essential for outer membrane targeting of DotH and Dot/Icm T4BSS core complex formation. These findings uncover an intriguing link between T4BSS and T2SS/T3SS.

 

Crystal structure of Legionella DotD: insights into the relationship between type IVB and type II/III secretion systems.,Nakano N, Kubori T, Kinoshita M, Imada K, Nagai H PLoS Pathog. 2010 Oct 7;6(10). pii: e1001129. PMID:20949065<ref>PMID:20949065</ref>

 

<references/>

[[Category: Legph]]

[[Category: Imada, K]]

[[Category: Kinoshita, M]]

[[Category: Kubori, T]]

[[Category: Nagai, H]]

[[Category: Nakano, N]]

[[Category: Proton transport]]