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| | ==Crystal Structure Analysis of the CofA== | | ==Crystal Structure Analysis of the CofA== |
| - | <StructureSection load='3vor' size='340' side='right' caption='[[3vor]], [[Resolution|resolution]] 0.90Å' scene=''> | + | <StructureSection load='3vor' size='340' side='right'caption='[[3vor]], [[Resolution|resolution]] 0.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3vor]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VOR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VOR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3vor]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VOR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VOR FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cofA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.9Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vor OCA], [http://pdbe.org/3vor PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vor RCSB], [http://www.ebi.ac.uk/pdbsum/3vor PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vor ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vor OCA], [https://pdbe.org/3vor PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vor RCSB], [https://www.ebi.ac.uk/pdbsum/3vor PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vor ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q59393_ECOLX Q59393_ECOLX] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Pilin|Pilin]] | + | *[[Pilin 3D structures|Pilin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus coli migula 1895]] | + | [[Category: Escherichia coli]] |
| - | [[Category: Baba, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Fukakusa, S]] | + | [[Category: Baba S]] |
| - | [[Category: Honda, T]] | + | [[Category: Fukakusa S]] |
| - | [[Category: Iida, T]] | + | [[Category: Honda T]] |
| - | [[Category: Iwasita, T]] | + | [[Category: Iida T]] |
| - | [[Category: Kawahara, K]] | + | [[Category: Iwasita T]] |
| - | [[Category: Kobayashi, Y]] | + | [[Category: Kawahara K]] |
| - | [[Category: Nakamura, S]] | + | [[Category: Kobayashi Y]] |
| - | [[Category: Nishimura, M]] | + | [[Category: Nakamura S]] |
| - | [[Category: Ohkubo, T]] | + | [[Category: Nishimura M]] |
| - | [[Category: Taniguchi, T]] | + | [[Category: Ohkubo T]] |
| - | [[Category: Cell adhesion]]
| + | [[Category: Taniguchi T]] |
| - | [[Category: Colonization]]
| + | |
| - | [[Category: Type iv pilin]]
| + | |
| Structural highlights
Function
Q59393_ECOLX
Publication Abstract from PubMed
CofA, a major pilin subunit of colonization factor antigen III (CFA/III), forms pili that mediate small-intestinal colonization by enterotoxigenic Escherichia coli (ETEC). In this study, the crystal structure of an N-terminally truncated version of CofA was determined by single-wavelength anomalous diffraction (SAD) phasing using five sulfurs in the protein. Given the counterbalance between anomalous signal strength and the undesired X-ray absorption of the solvent, diffraction data were collected at 1.5 A resolution using synchrotron radiation. These data were sufficient to elucidate the sulfur substructure at 1.38 A resolution. The low solvent content (29%) of the crystal necessitated that density modification be performed with an additional 0.9 A resolution data set to reduce the phase error caused by the small sulfur anomalous signal. The CofA structure showed the alphabeta-fold typical of type IVb pilins and showed high structural homology to that of TcpA for toxin-coregulated pili of Vibrio cholerae, including spatial distribution of key residues critical for pilin self-assembly. A pilus-filament model of CofA was built by computational docking and molecular-dynamics simulation using the previously reported filament model of TcpA as a structural template. This model revealed that the CofA filament surface was highly negatively charged and that a 23-residue-long loop between the alpha1 and alpha2 helices filled the gap between the pilin subunits. These characteristics could provide a unique binding epitope for the CFA/III pili of ETEC compared with other type IVb pili.
Structure of the CFA/III major pilin subunit CofA from human enterotoxigenic Escherichia coli determined at 0.90 A resolution by sulfur-SAD phasing.,Fukakusa S, Kawahara K, Nakamura S, Iwashita T, Baba S, Nishimura M, Kobayashi Y, Honda T, Iida T, Taniguchi T, Ohkubo T Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1418-29. Epub 2012 Sep, 13. PMID:22993096[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Fukakusa S, Kawahara K, Nakamura S, Iwashita T, Baba S, Nishimura M, Kobayashi Y, Honda T, Iida T, Taniguchi T, Ohkubo T. Structure of the CFA/III major pilin subunit CofA from human enterotoxigenic Escherichia coli determined at 0.90 A resolution by sulfur-SAD phasing. Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1418-29. Epub 2012 Sep, 13. PMID:22993096 doi:10.1107/S0907444912034464
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