This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4fs8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:23, 1 March 2024) (edit) (undo)
 
(One intermediate revision not shown.)
Line 1: Line 1:
==The structure of an As(III) S-adenosylmethionine methyltransferase: insights into the mechanism of arsenic biotransformation==
==The structure of an As(III) S-adenosylmethionine methyltransferase: insights into the mechanism of arsenic biotransformation==
-
<StructureSection load='4fs8' size='340' side='right' caption='[[4fs8]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
+
<StructureSection load='4fs8' size='340' side='right'caption='[[4fs8]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4fs8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cyanidioschyzon_sp._5508 Cyanidioschyzon sp. 5508]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3p7e 3p7e]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FS8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FS8 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4fs8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cyanidioschyzon_sp._5508 Cyanidioschyzon sp. 5508]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3p7e 3p7e]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FS8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FS8 FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78&#8491;</td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fr0|4fr0]], [[4fsd|4fsd]]</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">arsM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=610260 Cyanidioschyzon sp. 5508])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fs8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fs8 OCA], [https://pdbe.org/4fs8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fs8 RCSB], [https://www.ebi.ac.uk/pdbsum/4fs8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fs8 ProSAT]</span></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fs8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fs8 OCA], [http://pdbe.org/4fs8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fs8 RCSB], [http://www.ebi.ac.uk/pdbsum/4fs8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fs8 ProSAT]</span></td></tr>
+
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/C0JV69_9RHOD C0JV69_9RHOD]
-
Enzymatic methylation of arsenic is a detoxification process in microorganisms but in humans may activate the metalloid to more carcinogenic species. We describe the first structure of an As(III) S-adenosylmethionine methyltransferase by X-ray crystallography that reveals a novel As(III) binding domain. The structure of the methyltransferase from the thermophilic eukaryotic alga Cyanidioschyzon merolae reveals the relationship between the arsenic and S-adenosylmethionine binding sites to a final resolution of approximately 1.6 A. As(III) binding causes little change in conformation, but binding of SAM reorients helix alpha4 and a loop (residues 49-80) toward the As(III) binding domain, positioning the methyl group for transfer to the metalloid. There is no evidence of a reductase domain. These results are consistent with previous suggestions that arsenic remains trivalent during the catalytic cycle. A homology model of human As(III) S-adenosylmethionine methyltransferase with the location of known polymorphisms was constructed. The structure provides insights into the mechanism of substrate binding and catalysis.
+
-
 
+
-
Structure of an As(III) S-Adenosylmethionine Methyltransferase: Insights into the Mechanism of Arsenic Biotransformation.,Ajees AA, Marapakala K, Packianathan C, Sankaran B, Rosen BP Biochemistry. 2012 Jun 29. PMID:22712827<ref>PMID:22712827</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 4fs8" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Cyanidioschyzon sp. 5508]]
[[Category: Cyanidioschyzon sp. 5508]]
-
[[Category: Ajees, A A]]
+
[[Category: Large Structures]]
-
[[Category: Marapakala, K]]
+
[[Category: Ajees AA]]
-
[[Category: Packianathan, C]]
+
[[Category: Marapakala K]]
-
[[Category: Rosen, B P]]
+
[[Category: Packianathan C]]
-
[[Category: Sankaran, B]]
+
[[Category: Rosen BP]]
-
[[Category: Arsenic methyltransferase]]
+
[[Category: Sankaran B]]
-
[[Category: Rossmann fold]]
+
-
[[Category: Transferase]]
+

Current revision

The structure of an As(III) S-adenosylmethionine methyltransferase: insights into the mechanism of arsenic biotransformation

PDB ID 4fs8

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4fs8"
Personal tools