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| | ==Crystal structure of Staphylococcal Superantigen-Like protein 4 complexed with sialyl Lewis X== | | ==Crystal structure of Staphylococcal Superantigen-Like protein 4 complexed with sialyl Lewis X== |
| - | <StructureSection load='4dxg' size='340' side='right' caption='[[4dxg]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='4dxg' size='340' side='right'caption='[[4dxg]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4dxg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staae Staae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DXG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DXG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4dxg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_str._Newman Staphylococcus aureus subsp. aureus str. Newman]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DXG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DXG FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PIN:PIPERAZINE-N,N-BIS(2-ETHANESULFONIC+ACID)'>PIN</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=PIN:PIPERAZINE-N,N-BIS(2-ETHANESULFONIC+ACID)'>PIN</scene>, <scene name='pdbligand=PRD_900122:Sialyl-Lewis+X+antigen,+alpha+anomer'>PRD_900122</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4dxf|4dxf]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dxg OCA], [https://pdbe.org/4dxg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dxg RCSB], [https://www.ebi.ac.uk/pdbsum/4dxg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dxg ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NWMN_0391, ssl4nm ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=426430 STAAE])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dxg OCA], [http://pdbe.org/4dxg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dxg RCSB], [http://www.ebi.ac.uk/pdbsum/4dxg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dxg ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/SSL4_STAAE SSL4_STAAE] Secreted protein that plays a role in immune innate response inhibition by interfering with host TLR2-mediated pathway.[UniProtKB:Q2G1S8] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Staae]] | + | [[Category: Large Structures]] |
| - | [[Category: Baker, E N]] | + | [[Category: Staphylococcus aureus subsp. aureus str. Newman]] |
| - | [[Category: Baker, H M]] | + | [[Category: Baker EN]] |
| - | [[Category: Fraser, J F]] | + | [[Category: Baker HM]] |
| - | [[Category: Hermans, S J]] | + | [[Category: Fraser JF]] |
| - | [[Category: Langley, R J]] | + | [[Category: Hermans SJ]] |
| - | [[Category: Beta grasp fold]] | + | [[Category: Langley RJ]] |
| - | [[Category: Carbohydrate binding]]
| + | |
| - | [[Category: Oligonucleotide/oligosaccharide binding fold]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| - | [[Category: Superantigen fold]]
| + | |
| Structural highlights
4dxg is a 1 chain structure with sequence from Staphylococcus aureus subsp. aureus str. Newman. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 2.5Å |
| Ligands: | , , , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
SSL4_STAAE Secreted protein that plays a role in immune innate response inhibition by interfering with host TLR2-mediated pathway.[UniProtKB:Q2G1S8]
Publication Abstract from PubMed
Staphylococcus aureus is a prevalent and significant human pathogen. Among the repertoire of virulence factors produced by this bacterium are the 14 Staphylococcal superantigen-like (SSL) proteins. SSL4 is one member of this family and contains a highly conserved carbohydrate binding site also found in SSL2, SSL3, SSL5, SSL6, and SSL11. Recombinant SSL4(t), comprising amino acids 109-309 of SSL4-Newman, is shown to bind and be internalised by human granulocytes and macrophages in a sialic acid (Sia) dependent manner. SSL4(t) can compete with itself for cell binding, indicating that binding is target specific. A 2.5A resolution crystal structure of SSL4(t) complexed with sialyl Lewis-X (sLe(x) - Neu5Acalpha2-3Galbeta1-4(Fucalpha1-3)GlcNAc) revealed a similar binding site to SSL5 and SSL11. These data, along with data on SSL4(t) binding to a glycan array and biosensor analysis of sLe(x) and sLacNac binding are compared with SSL11. Although these proteins show great similarity in their carbohydrate binding sites with a root mean square (rms) difference between main chain atom positions of only 0.34A, these proteins differ in detail in their affinity for sLe(x) and sLacNac, as well as their glycan preference. Together with cell binding data this shows how S. aureus produces multiple related proteins that target myeloid cells through specific sialyllactosamine containing glycoproteins.
The Structural and Functional Properties of Staphylococcal Superantigen-Like 4 (SSL4).,Hermans SJ, Baker HM, Sequeira RP, Langley RJ, Baker EN, Fraser JD Infect Immun. 2012 Sep 4. PMID:22949551[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hermans SJ, Baker HM, Sequeira RP, Langley RJ, Baker EN, Fraser JD. The Structural and Functional Properties of Staphylococcal Superantigen-Like 4 (SSL4). Infect Immun. 2012 Sep 4. PMID:22949551 doi:http://dx.doi.org/10.1128/IAI.00764-12
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