1nox

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[[Image:1nox.gif|left|200px]]
 
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{{Structure
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==NADH OXIDASE FROM THERMUS THERMOPHILUS==
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|PDB= 1nox |SIZE=350|CAPTION= <scene name='initialview01'>1nox</scene>, resolution 1.59&Aring;
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<StructureSection load='1nox' size='340' side='right'caption='[[1nox]], [[Resolution|resolution]] 1.59&Aring;' scene=''>
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|SITE= <scene name='pdbsite=COF:Cofactor'>COF</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>
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<table><tr><td colspan='2'>[[1nox]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NOX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NOX FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/NADH_dehydrogenase NADH dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.3 1.6.99.3] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.59&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nox FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nox OCA], [https://pdbe.org/1nox PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nox RCSB], [https://www.ebi.ac.uk/pdbsum/1nox PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nox ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nox FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nox OCA], [http://www.ebi.ac.uk/pdbsum/1nox PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nox RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/NOX_THET8 NOX_THET8] Can oxidize either NADH or NADPH with a preference for NADH. Can catalyze electron transfer from NADH to various electron acceptors which include, in addition to molecular oxygen, cytochrome c, 2,6 dichlorphenolindophenol, methylene blue, ferricyanide or P-nitroblue tetrazolium.
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== Evolutionary Conservation ==
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'''NADH OXIDASE FROM THERMUS THERMOPHILUS'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/no/1nox_consurf.spt"</scriptWhenChecked>
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The crystal structures of the flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) containing isoforms of NADH oxidase from Thermus thermophilus have been determined by isomorphous and molecular replacement and refined to 2.3 A and 1.6 A resolution with R-values of 18.5% and 18.6% respectively. The structure of the homodimeric enzyme consists of a central 4-stranded antiparallel beta-sheet covered by helices, a more flexible domain formed by two helices, and a C-terminal excursion connecting the subunits. The active sites are located in a deep cleft between the subunits. The binding site of the flavin cofactor lacks the common nucleotide binding fold and is different from the FMN binding site found in flavodoxins.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1NOX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NOX OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nox ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Crystal structure of NADH oxidase from Thermus thermophilus., Hecht HJ, Erdmann H, Park HJ, Sprinzl M, Schmid RD, Nat Struct Biol. 1995 Dec;2(12):1109-14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8846223 8846223]
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</StructureSection>
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[[Category: NADH dehydrogenase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Thermus thermophilus]]
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[[Category: Erdmann H]]
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[[Category: Erdmann, H.]]
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[[Category: Hecht HJ]]
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[[Category: Hecht, H J.]]
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[[Category: Park HJ]]
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[[Category: Park, H J.]]
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[[Category: Schmid RD]]
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[[Category: Schmid, R D.]]
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[[Category: Sprinzl M]]
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[[Category: Sprinzl, M.]]
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[[Category: flavoenzyme]]
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[[Category: flavoprotein fmn]]
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[[Category: oxidoreductase]]
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[[Category: thermophile]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:33:24 2008''
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Current revision

NADH OXIDASE FROM THERMUS THERMOPHILUS

PDB ID 1nox

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