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| | ==GlnK2 from Haloferax mediterranei complexed with AMP== | | ==GlnK2 from Haloferax mediterranei complexed with AMP== |
| - | <StructureSection load='4ozl' size='340' side='right' caption='[[4ozl]], [[Resolution|resolution]] 1.49Å' scene=''> | + | <StructureSection load='4ozl' size='340' side='right'caption='[[4ozl]], [[Resolution|resolution]] 1.49Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ozl]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OZL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ozl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haloferax_mediterranei_ATCC_33500 Haloferax mediterranei ATCC 33500]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OZL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OZL FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4942Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ozl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ozl OCA], [http://pdbe.org/4ozl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ozl RCSB], [http://www.ebi.ac.uk/pdbsum/4ozl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ozl ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ozl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ozl OCA], [https://pdbe.org/4ozl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ozl RCSB], [https://www.ebi.ac.uk/pdbsum/4ozl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ozl ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/GLNK2_HALMT GLNK2_HALMT] Involved in the regulation of nitrogen metabolism (PubMed:21265784, PubMed:23420616). Regulates the activity of its targets by protein-protein interaction in response to the nitrogen status of the cell (PubMed:23069245). Increases the activity of the glutamine synthetase 3 in the presence of 2-oxoglutarate (PubMed:23069245). May regulate the activity of the ammonia channel Amt2 via direct interaction (By similarity).[UniProtKB:B8ZYW0]<ref>PMID:21265784</ref> <ref>PMID:23069245</ref> <ref>PMID:23420616</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bonete, M J]] | + | [[Category: Haloferax mediterranei ATCC 33500]] |
| - | [[Category: Camacho, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Llacer, J L]] | + | [[Category: Bonete MJ]] |
| - | [[Category: Palanca, C]] | + | [[Category: Camacho M]] |
| - | [[Category: Pedro-Roig, L]] | + | [[Category: Llacer JL]] |
| - | [[Category: Rubio, V]] | + | [[Category: Palanca C]] |
| - | [[Category: Archaea]] | + | [[Category: Pedro-Roig L]] |
| - | [[Category: Glnb]] | + | [[Category: Rubio V]] |
| - | [[Category: Glnk]]
| + | |
| - | [[Category: Haloferax mediterranei]]
| + | |
| - | [[Category: Halophile]]
| + | |
| - | [[Category: Pii]]
| + | |
| - | [[Category: Signaling]]
| + | |
| Structural highlights
Function
GLNK2_HALMT Involved in the regulation of nitrogen metabolism (PubMed:21265784, PubMed:23420616). Regulates the activity of its targets by protein-protein interaction in response to the nitrogen status of the cell (PubMed:23069245). Increases the activity of the glutamine synthetase 3 in the presence of 2-oxoglutarate (PubMed:23069245). May regulate the activity of the ammonia channel Amt2 via direct interaction (By similarity).[UniProtKB:B8ZYW0][1] [2] [3]
Publication Abstract from PubMed
To get insight on archaeal nitrogen signaling and on haloadaptation of the nitrogen/carbon/energy-signaling protein PII, we determined crystal structures of recombinantly produced GlnK2 from the extreme halophilic archaea Haloferax mediterranei, complexed with AMP or with the PII effectors ADP or ATP, at respective resolutions of 1.49, 1.45 and 2.60 A. A unique trait of these structures was a three-tonged crown protruding from the trimer body convex side, formed by an 11-residue, N-terminal, highly acidic extension that is absent from structurally studied PII proteins. This extension substantially contributed to the very low pI value which is an haloadaptive trait of H.mediterranei GlnK2, and participated in hexamer-forming contacts in one crystal. Similar acidic N-extensions are shown here to be common among PII proteins from halophilic organisms. Additional haloadaptive traits prominently represented in H. mediterranei GlnK2 are a very high ratio of small residues versus large hydrophobic aliphatic residues; and the largest ratio of polar to non-polar exposed surface for any structurally characterized PII protein. The presence of a dense hydration layer in the region between the three T-loops might also be a haloadaptation. Other unique findings revealed by the GlnK2 structure that might have functional relevance are the adoption by its T-loop of a 3-turn alpha helical conformation, perhaps in relation with the GlnK2 ability to directly interact with glutamine synthetase; and the firm binding of AMP, confirmed by biochemical binding studies with ATP, ADP and AMP, raising the possibility that AMP could be an important PII effector at least in Archaea. This article is protected by copyright. All rights reserved.
The structure of a PII signaling protein from a halophilic archaea reveals novel traits and high-salt adaptations.,Palanca C, Pedro-Roig L, Llacer JL, Camacho M, Bonete MJ, Rubio V FEBS J. 2014 Jun 20. doi: 10.1111/febs.12881. PMID:24946894[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Pedro-Roig L, Camacho M, Bonete MJ. In vitro proof of direct regulation of glutamine synthetase by GlnK proteins in the extreme halophilic archaeon Haloferax mediterranei. Biochem Soc Trans. 2011 Jan;39(1):259-62. doi: 10.1042/BST0390259. PMID:21265784 doi:http://dx.doi.org/10.1042/BST0390259
- ↑ Pedro-Roig L, Camacho M, Bonete MJ. Regulation of ammonium assimilation in Haloferax mediterranei: interaction between glutamine synthetase and two GlnK proteins. Biochim Biophys Acta. 2013 Jan;1834(1):16-23. doi: 10.1016/j.bbapap.2012.10.006. , Epub 2012 Oct 12. PMID:23069245 doi:http://dx.doi.org/10.1016/j.bbapap.2012.10.006
- ↑ Pedro-Roig L, Camacho M, Bonete MJ. Haloferax mediterranei GlnK proteins are post-translationally modified by uridylylation. Proteomics. 2013 Apr;13(8):1371-4. doi: 10.1002/pmic.201200465. Epub 2013 Mar 18. PMID:23420616 doi:http://dx.doi.org/10.1002/pmic.201200465
- ↑ Palanca C, Pedro-Roig L, Llacer JL, Camacho M, Bonete MJ, Rubio V. The structure of a PII signaling protein from a halophilic archaea reveals novel traits and high-salt adaptations. FEBS J. 2014 Jun 20. doi: 10.1111/febs.12881. PMID:24946894 doi:http://dx.doi.org/10.1111/febs.12881
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