This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4fdx

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:15, 1 March 2024) (edit) (undo)
 
(One intermediate revision not shown.)
Line 1: Line 1:
==Kinetic and structural characterization of the 4-oxalocrotonate tautomerase isozymes from Methylibium petroleiphilum==
==Kinetic and structural characterization of the 4-oxalocrotonate tautomerase isozymes from Methylibium petroleiphilum==
-
<StructureSection load='4fdx' size='340' side='right' caption='[[4fdx]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
+
<StructureSection load='4fdx' size='340' side='right'caption='[[4fdx]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4fdx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Metpp Metpp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FDX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FDX FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4fdx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylibium_petroleiphilum_PM1 Methylibium petroleiphilum PM1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FDX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FDX FirstGlance]. <br>
-
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4faz|4faz]]</td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.64&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mpe_A3323 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=420662 METPP])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fdx OCA], [https://pdbe.org/4fdx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fdx RCSB], [https://www.ebi.ac.uk/pdbsum/4fdx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fdx ProSAT]</span></td></tr>
-
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oxaloacetate_tautomerase Oxaloacetate tautomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.2.2 5.3.2.2] </span></td></tr>
+
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fdx OCA], [http://pdbe.org/4fdx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fdx RCSB], [http://www.ebi.ac.uk/pdbsum/4fdx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fdx ProSAT]</span></td></tr>
+
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/A2SL37_METPP A2SL37_METPP]
-
Methylibium petroleiphilum strain PM1 uses various petroleum products including the fuel additive methyl tert-butyl ether and straight chain and aromatic hydrocarbons as sole carbon and energy sources. It has two operons, dmpI and dmpII, that code for the enzymes in a pair of parallel meta-fission pathways. In order to understand the roles of the pathways, the 4-oxalocrotonate tautomerase (4-OT) isozyme from each pathway was characterized. Tautomerase I and tautomerase II have the lowest pairwise sequence identity (35%) among the isozyme pairs in the parallel pathways, and could offer insight into substrate preferences and pathway functions. The kinetic parameters of tautomerase I and tautomerase II were determined using 2-hydroxymuconate and 5-(methyl)-2-hydroxymuconate. Both tautomerase I and tautomerase II process the substrates, but with different efficiencies. Crystal structures were determined for both tautomerase I and tautomerase II, at 1.57 and 1.64A resolution, respectively. The backbones of tautomerase I and tautomerase II are highly similar, but have distinct active site environments. The results, in combination with those for other structurally and kinetically characterized 4-OT isozymes, suggest that tautomerase I catalyzes the tautomerization of both 2-hydroxymuconate and alkyl derivatives, whereas tautomerase II might specialize in other aromatic hydrocarbon metabolites.
+
-
 
+
-
Structural and kinetic characterization of two 4-oxalocrotonate tautomerases in Methylibium petroleiphilum strain PM1.,Terrell CR, Burks EA, Whitman CP, Hoffman DW Arch Biochem Biophys. 2013 Sep 1;537(1):113-24. doi: 10.1016/j.abb.2013.06.016., Epub 2013 Jul 4. PMID:23831510<ref>PMID:23831510</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 4fdx" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Metpp]]
+
[[Category: Large Structures]]
-
[[Category: Oxaloacetate tautomerase]]
+
[[Category: Methylibium petroleiphilum PM1]]
-
[[Category: Hoffman, D W]]
+
[[Category: Hoffman DW]]
-
[[Category: Terrell, C R]]
+
[[Category: Terrell CR]]
-
[[Category: Whitman, C P]]
+
[[Category: Whitman CP]]
-
[[Category: Alpha/beta barrel]]
+
-
[[Category: Isomerase]]
+
-
[[Category: Tautomerase superfamily]]
+

Current revision

Kinetic and structural characterization of the 4-oxalocrotonate tautomerase isozymes from Methylibium petroleiphilum

PDB ID 4fdx

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4fdx"
Personal tools