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| | ==Crystal Structure of a Family GH19 Chitinase from Bryum coronatum in complex with (GlcNAc)4 at 1.0 A resolution== | | ==Crystal Structure of a Family GH19 Chitinase from Bryum coronatum in complex with (GlcNAc)4 at 1.0 A resolution== |
| - | <StructureSection load='3wh1' size='340' side='right' caption='[[3wh1]], [[Resolution|resolution]] 1.00Å' scene=''> | + | <StructureSection load='3wh1' size='340' side='right'caption='[[3wh1]], [[Resolution|resolution]] 1.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3wh1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bryum_coronatum Bryum coronatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WH1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WH1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3wh1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gemmabryum_coronatum Gemmabryum coronatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WH1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WH1 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ij4|4ij4]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bcchiA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216087 Bryum coronatum])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wh1 OCA], [https://pdbe.org/3wh1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wh1 RCSB], [https://www.ebi.ac.uk/pdbsum/3wh1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wh1 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wh1 OCA], [http://pdbe.org/3wh1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wh1 RCSB], [http://www.ebi.ac.uk/pdbsum/3wh1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wh1 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A9ZSX9_9BRYO A9ZSX9_9BRYO] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| | *[[Chitinase|Chitinase]] | | *[[Chitinase|Chitinase]] |
| | + | *[[Chitinase 3D structures|Chitinase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bryum coronatum]] | + | [[Category: Gemmabryum coronatum]] |
| - | [[Category: Chitinase]] | + | [[Category: Large Structures]] |
| - | [[Category: Fukamizo, T]] | + | [[Category: Fukamizo T]] |
| - | [[Category: Numata, T]] | + | [[Category: Numata T]] |
| - | [[Category: Ohnuma, T]] | + | [[Category: Ohnuma T]] |
| - | [[Category: Umemoto, N]] | + | [[Category: Umemoto N]] |
| - | [[Category: Carbohydrate]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
A9ZSX9_9BRYO
Publication Abstract from PubMed
DESCRIPTIONS: The structure of a GH19 chitinase from the moss Bryum coronatum (BcChi-A) in complex with the substrate was examined by X-ray crystallography and NMR spectroscopy in solution. The X-ray crystal structure of the inactive mutant of BcChi-A (BcChi-A-E61A) liganded with chitin tetramer (GlcNAc)4 revealed a clear electron density of the tetramer bound to subsites -2, -1, +1, and +2. Individual sugar residues were recognized by several amino acids at these subsites through a number of hydrogen bonds. This is the first crystal structure of GH19 chitinase liganded with oligosaccharide spanning the catalytic center. NMR titration experiments of chitin oligosaccharides into the BcChi-A-E61A solution showed that the binding mode observed in the crystal structure is similar to that in solution. The C-1 carbon of -1 GlcNAc, the Oepsilon1 atom of the catalytic base (Glu70), and the Ogamma atom of Ser102 form a "triangle" surrounding the catalytic water, and the arrangement structurally validated the proposed catalytic mechanism of GH19 chitinases. The glycosidic linkage between -1 and +1 sugars was found to be twisted and under strain. This situation may contribute to the reduction of activation energy for hydrolysis. The complex structure revealed a more refined mechanism of the chitinase catalysis.
Crystal structure of a "loopless" GH19 chitinase in complex with chitin tetrasaccharide spanning the catalytic center.,Ohnuma T, Umemoto N, Nagata T, Shinya S, Numata T, Taira T, Fukamizo T Biochim Biophys Acta. 2014 Feb 25;1844(4):793-802. doi:, 10.1016/j.bbapap.2014.02.013. PMID:24582745[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ohnuma T, Umemoto N, Nagata T, Shinya S, Numata T, Taira T, Fukamizo T. Crystal structure of a "loopless" GH19 chitinase in complex with chitin tetrasaccharide spanning the catalytic center. Biochim Biophys Acta. 2014 Feb 25;1844(4):793-802. doi:, 10.1016/j.bbapap.2014.02.013. PMID:24582745 doi:http://dx.doi.org/10.1016/j.bbapap.2014.02.013
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